Mycobaterium leprae gyrA conferring resistance to fluoroquinolones

Accession ARO:3003298
DefinitionPoint mutation of Mycobaterium leprae gyrA resulted in the lowered affinity between fluoroquinolones and gyrA. Thus, conferring resistance.
AMR Gene Familyfluoroquinolone resistant gyrA
Drug Classfluoroquinolone antibiotic
Resistance Mechanismantibiotic target alteration
Classification12 ontology terms | Show
Parent Term(s)14 ontology terms | Show
+ fluoroquinolone resistant gyrA [AMR Gene Family]
+ confers_resistance_to_antibiotic ciprofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic enoxacin [Antibiotic]
+ confers_resistance_to_antibiotic gatifloxacin [Antibiotic]
+ confers_resistance_to_antibiotic grepafloxacin [Antibiotic]
+ confers_resistance_to_antibiotic levofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic lomefloxacin [Antibiotic]
+ confers_resistance_to_antibiotic moxifloxacin [Antibiotic]
+ confers_resistance_to_antibiotic nalidixic acid [Antibiotic]
+ confers_resistance_to_antibiotic norfloxacin [Antibiotic]
+ confers_resistance_to_antibiotic ofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic pefloxacin [Antibiotic]
+ confers_resistance_to_antibiotic sparfloxacin [Antibiotic]
+ confers_resistance_to_antibiotic trovafloxacin [Antibiotic]
Publications

You EY, et al. 2004. J Infect 50(1): 6-11. Mutations in genes related to drug resistance in Mycobacterium leprae isolates from leprosy patients in Korea. (PMID 15603834)

Resistomes

Prevalence of Mycobaterium leprae gyrA conferring resistance to fluoroquinolones among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
No prevalence data


Detection Models

Model Type: protein variant model

Model Definition: The protein variant model is an AMR detection model. Protein variant models are similar to protein homolog models - they detect the presence of a protein sequence based on its similarity to a curated reference sequence, but secondarily search submitted query sequences for curated sets of mutations shown clinically to confer resistance relative to wild-type. This model includes a protein reference sequence, a curated BLASTP cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of: single resistance variants, insertions, deletions, co-dependent resistance variants, nonsense SNPs, and/or frameshift mutations. Protein variant model matches to reference sequences are categorized on two criteria: strict and loose. A strict match has a BLASTP bitscore above the curated BLASTP cutoff value and contains at least one detected mutation from amongst the mapped resistance variants; a loose match has a BLASTP bitscore below the curated BLASTP cutoff value but still contains at least one detected mutation from amongst the mapped resistance variants. Regardless of BLASTP bitscore, if a sequence does not contain one of the mapped resistance variants, it is not considered a match and not detected by the protein variant model.

Legend:

  • discovered in clinical, agricultural, or environmental isolates
  • discovered via laboratory selection experiments


Bit-score Cut-off (blastP): 1500


>gb|CAC29514.1|+|Mycobaterium leprae gyrA conferring resistance to fluoroquinolones [Mycobacterium leprae TN]
MTDITLPPGDGSIQRVEPVDIQQEMQRSYIDYAMSVIVGRALPEVRDGLKPVHRRVLYAM
LDSGFRPDRSHAKSARSVAETMGNYHPHGDASIYDTLVRMAQPWSLRYPLVDGQGNFGSP
GNDPPAAMRYCVSGNSLVRLLFGKSIRIGDIVTGAQFNSDNPIDLKVLDRHGNPVVADYL
FHSGEHQTYTVRTTEGYEITGTSNHPLLCLVNVGGIPTLLWKLIGEIRSGDYVVLQRIPP
VEFGPADWYSTMEALLFGAFISGGFVFQDHAGFNSLDRDYFTMVVNAYDTVVGGLRCISS
RITVSGSTLLELDVYNLIEFKKTRLSGLCGQRSADKLVPDWLWHSPSTVKRAFLQALFEG
EGFSSILSRNIIEISYSTLSERLAADVQQMLLEFGVVSERYCHTVNEYKVVIANRAQVEM
FFTQVGFGVTKQAKLIRDVVSMSPCVGMDINCVPGLATFIRKHCDNRWVEEDSFNQHNVD
CVQHWHHHSAEIVGHIADPDIRAIVTDLTDGRFYYARVASVTDTGIQPVFSLHVDTEDHS
FLTNGFISHNTEARLTPLAMEMLREIDEETVDFISNYDGRVQEPMVLPSRFPNLLANGSG
GIAVGMATNIPPHNLYELADAVFWCLENHDADEETMLVAVMERVKGPDFPTAGLIVGSQG
IADAYKTGRGSIRIRGVVEVEEDSRGRTSLVITELPYQVNHDNFITSIAEQVRTGRLAGI
SNVEDQGSDRVGVRIVIEIKRDAVAKVVLNNLYKHTQLQTSFGANMLSIVDGVPRTLRLD
QMICYYVEHQLDVIVRRTTYRLRKANERAHILRGLVKALDALDEVITLIRASQTVDIARV
GVVELLDIDDIQAQAILDMQLRRLAALERQRIIDDLAKIEVEIADLGDILAKPERRRGII
RNELTEIAEKYGDDRRTRIIAVDGDVNDEDLIAREEVVVTITETGYAKRTKTDLYRSQKR
GGKGVQGAGLKQDDIVRHFFVCSTHDWILFFTTQGRVYRAKAYELPEASRTARGQHVANL
LAFQPEERIAQVIQIRSYEDAPYLVLATRAGLVKKSKLTDFDSNRSGGIVAINLRDNDEL
VGAVLCAADGDLLLVSANGQSIRFSATDEALRPMGRATSGVQGMRFNADDRLLSLNVVRE
DTYLLVATSGGYAKRTSIEEYPMQGRGGKGVLTVMYDRRRGSLVGAIVVDEDSELYAITS
GGGVIRTTARQVRQAGRQTKGVRLMNLGEGDTLLAIARNAEESADGGVG



>gb|AL450380|+|7318-11067|Mycobaterium leprae gyrA conferring resistance to fluoroquinolones [Mycobacterium leprae TN]
ATGACTGATATCACGCTGCCACCAGGTGACGGTTCTATACAGCGGGTTGAGCCGGTCGACATTCAGCAGGAAATGCAGCGCAGCTATATT
GATTACGCGATGAGTGTGATTGTGGGCCGGGCGTTGCCTGAAGTCCGCGATGGTCTCAAACCGGTACATCGTCGGGTCTTGTACGCGATG
TTAGACTCCGGTTTCCGCCCGGACCGTAGCCACGCTAAGTCAGCACGGTCAGTCGCTGAGACGATGGGCAATTACCATCCGCACGGCGAC
GCATCGATTTATGACACGTTAGTGCGCATGGCGCAGCCGTGGTCGCTGCGGTATCCCTTGGTTGATGGGCAAGGCAATTTCGGTTCGCCG
GGTAATGACCCACCGGCAGCGATGCGTTATTGTGTGTCAGGAAATTCCTTGGTGAGGTTGCTATTTGGGAAATCAATACGAATCGGTGAT
ATCGTTACTGGAGCTCAGTTCAATTCGGACAATCCGATCGACTTGAAGGTTCTTGATCGGCATGGTAATCCGGTTGTAGCCGATTATTTA
TTCCATTCAGGAGAGCACCAAACCTATACAGTGCGCACCACTGAAGGCTATGAGATCACCGGGACGTCGAACCATCCCTTGTTGTGTTTA
GTGAATGTCGGCGGTATACCCACCTTGTTGTGGAAGCTGATTGGAGAAATTCGATCAGGAGACTACGTTGTTTTACAGCGGATCCCACCA
GTGGAATTTGGTCCGGCGGACTGGTATTCTACGATGGAAGCATTGTTATTCGGAGCCTTTATTAGTGGGGGCTTCGTTTTTCAGGACCAT
GCTGGATTTAACAGCCTTGACCGTGACTATTTCACCATGGTTGTTAATGCTTATGATACGGTTGTGGGTGGCCTGCGTTGCATATCTTCT
CGAATCACCGTATCGGGGTCGACGCTACTCGAACTTGATGTTTATAACCTCATCGAGTTTAAGAAGACAAGACTTAGCGGTTTATGCGGG
CAACGGTCTGCGGACAAGTTGGTACCTGACTGGTTGTGGCACTCACCTTCCACCGTCAAACGAGCATTCCTTCAGGCATTGTTTGAAGGT
GAAGGATTTTCTTCGATATTGTCGCGAAATATAATTGAGATTTCCTACTCGACACTTAGTGAGCGACTGGCCGCCGACGTCCAGCAGATG
CTGCTTGAATTCGGAGTCGTGTCTGAGCGCTATTGCCATACTGTCAATGAGTACAAGGTTGTCATAGCTAACCGCGCTCAAGTAGAAATG
TTTTTCACCCAAGTCGGTTTCGGTGTTACTAAACAAGCTAAGCTTATCCGGGACGTGGTATCTATGTCTCCATGCGTTGGCATGGATATC
AACTGCGTACCAGGTTTGGCCACTTTCATTCGTAAGCATTGTGATAACCGCTGGGTCGAGGAAGACTCATTTAATCAGCATAATGTTGAT
TGCGTCCAACATTGGCACCATCATAGCGCGGAAATCGTCGGCCACATCGCCGATCCCGATATTCGTGCCATCGTGACTGACCTTACTGAT
GGCCGGTTCTACTACGCGCGCGTCGCGTCCGTGACTGATACCGGTATTCAACCTGTGTTCAGTCTACATGTGGACACCGAGGATCATTCG
TTTTTGACTAATGGATTCATCAGCCATAACACCGAGGCTCGGCTTACTCCATTGGCGATGGAAATGTTGCGCGAGATCGACGAGGAGACA
GTTGATTTCATATCTAACTACGATGGCCGGGTGCAGGAACCGATGGTGTTGCCTAGCCGTTTTCCCAACCTGTTGGCTAATGGTTCTGGC
GGTATCGCGGTCGGCATGGCTACCAATATCCCGCCGCACAACCTGTATGAGCTCGCCGACGCTGTGTTTTGGTGCCTAGAGAACCATGAC
GCTGACGAAGAGACGATGCTGGTCGCTGTTATGGAACGGGTCAAAGGTCCTGATTTCCCTACCGCCGGGTTGATTGTCGGTTCGCAAGGC
ATTGCCGATGCTTACAAGACTGGCCGTGGTTCCATTCGGATACGCGGAGTTGTTGAGGTTGAAGAAGATTCACGCGGAAGGACGTCATTG
GTCATCACTGAGCTACCGTATCAGGTCAACCACGACAACTTCATCACTTCTATCGCTGAGCAAGTCCGCACTGGCCGGCTAGCCGGCATC
TCCAATGTAGAAGACCAAGGCAGCGACCGGGTTGGTGTACGTATCGTCATCGAGATCAAGCGTGACGCGGTGGCCAAAGTGGTGCTCAAT
AACCTGTACAAGCATACTCAGCTGCAAACTAGTTTCGGAGCCAACATGTTGTCAATCGTTGACGGCGTGCCGCGCACTTTGCGGTTGGAT
CAGATGATTTGTTATTATGTCGAACATCAACTGGACGTCATTGTCCGGCGCACTACCTACCGATTGCGTAAAGCCAACGAGCGGGCTCAT
ATTTTGCGTGGATTGGTCAAAGCGCTCGATGCGTTAGATGAGGTTATTACGTTGATTCGGGCATCGCAGACCGTGGATATTGCTCGTGTT
GGGGTGGTCGAGTTACTCGATATCGACGACATTCAGGCTCAAGCTATCCTGGACATGCAGCTGCGGCGTTTGGCGGCTTTGGAGCGTCAA
CGCATTATTGATGATCTCGCTAAGATTGAGGTCGAGATCGCTGATCTGGGAGATATTCTGGCTAAGCCGGAGCGTCGGCGTGGTATCATT
CGTAATGAACTGACTGAGATCGCAGAGAAGTACGGTGATGACCGTCGTACTCGGATAATAGCGGTTGATGGTGATGTCAACGACGAGGAT
TTGATTGCTCGTGAAGAGGTCGTTGTCACGATAACTGAAACTGGATATGCTAAACGTACTAAAACTGACCTGTATCGCAGCCAGAAACGC
GGCGGGAAAGGTGTTCAAGGCGCCGGTTTGAAGCAGGACGACATCGTCCGGCATTTCTTCGTGTGTTCAACTCACGATTGGATCCTGTTT
TTCACCACCCAAGGCCGCGTATACCGGGCCAAGGCCTATGAATTGCCAGAGGCTTCTCGAACGGCACGCGGGCAACACGTGGCCAATTTG
CTTGCATTCCAGCCTGAAGAGCGCATCGCTCAGGTAATTCAGATCCGTAGCTATGAAGACGCTCCATACTTGGTCCTTGCCACGCGCGCC
GGTCTGGTTAAGAAGTCAAAGTTGACCGATTTTGACTCTAATCGTTCGGGTGGGATCGTGGCAATTAATTTACGTGACAACGATGAGTTG
GTCGGTGCAGTGTTGTGCGCGGCCGACGGCGACTTGCTTCTGGTATCGGCTAACGGCCAGTCTATCCGGTTCTCAGCGACTGACGAGGCC
TTGCGTCCGATGGGGCGGGCTACCTCTGGTGTGCAGGGCATGCGGTTTAACGCCGATGATCGACTGTTGTCGTTGAATGTGGTTCGCGAA
GATACTTACCTGCTTGTCGCAACGTCTGGGGGTTACGCTAAACGCACCTCGATTGAGGAGTACCCGATGCAGGGCCGTGGCGGAAAGGGT
GTTCTAACGGTCATGTACGATCGTCGGCGCGGTAGCTTGGTTGGGGCCATCGTGGTTGATGAAGACAGCGAGTTGTACGCGATCACCTCA
GGGGGTGGGGTAATTCGTACAACGGCACGCCAGGTTCGCCAGGCAGGACGCCAGACCAAGGGTGTTCGGTTGATGAACTTAGGTGAGGGC
GACACGCTGTTAGCCATCGCACGTAATGCCGAAGAAAGCGCCGACGGCGGTGTCGGTTAA