MCR-3.3

Accession ARO:3004662
CARD Short NameMCR-3.3
DefinitionMCR-3.3 is a colistin resistance gene variant in the chromosome of an Aeromonas veronii. Conferred polymyxin resistance in both E. Coli and Aeromonas salmonicida.
AMR Gene FamilyMCR phosphoethanolamine transferase
Drug Classpeptide antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Perfect MatchesAeromonas caviaewgs, Aeromonas veroniig+wgs
Resistomes with Sequence VariantsAeromonas caviaewgs, Aeromonas veroniig+wgs
Classification14 ontology terms | Show
Parent Term(s)4 ontology terms | Show
+ MCR phosphoethanolamine transferase [AMR Gene Family]
+ evolutionary_variant_of MCR-3.1
+ confers_resistance_to_antibiotic colistin A [Antibiotic]
+ confers_resistance_to_antibiotic colistin B [Antibiotic]
Publications

Ling Z, et al. 2017. Antimicrob. Agents Chemother. 61(11): Chromosome-Mediated mcr-3 Variants in Aeromonas veronii from Chicken Meat. (PMID 28848017)

Resistomes

Prevalence of MCR-3.3 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Aeromonas caviae0%0%0.54%0%
Aeromonas veronii1.82%0%2.81%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 1010


>gb|ASU10319.1|-|MCR-3.3 [Aeromonas veronii]
MPSLIKIKIVPLMFFLALYFAFMLNWRGVLHFYEILYKLEDFKFGFAISLPILLVAALNFVFVPFSIRYLIKPFFALLIALSAIVSYTMM
KYRVLFDQNMIQNIFETNQNEALAYLSLPIIGWVTIAGFIPAILLFFVEIEYEEKWFKGILTRALSMFASLIVIAVIAALYYQDYVSVGR
NNSNLQREIVPANFVNSTVKYVYNRYLAEPIPFTTLGDDAKRDTNQSKPTLMFLVVGETARGKNFSMNGYEKDTNPFTSKSGGVISFNDV
RSCGTATAVSVPCMFSNMGRKEFDDNLARNSEGLLDVLQKTGVSIFWKENDGGCKGVCDRVPNIEIKPKDYPKFCDKNTCYDEVVLQELD
SEIAQMKGDKLVGFHLIGSHGPTYYKRYPDAHRQFTPDCPRSDIENCTDEELTNTYDNTIRYTDFVIAEMIAKLKTYEDKYNTALLYVSD
HGESLGALGLYLHGTPYKFAPDDQTRVPMQVWMSPGFIKEKGMNMECLQKNAAANRYSHDNIFSSVLGIWDVKTAIYEQELDIFKQCRNN


>gb|MF495680.1|-|240179-241801|MCR-3.3 [Aeromonas veronii]
ATGCCTTCCCTTATAAAAATAAAAATTGTTCCGCTTATGTTCTTTTTGGCACTGTATTTTGCATTTATGCTGAACTGGCGTGGAGTTCTC
CATTTTTACGAAATCCTTTACAAATTAGAAGATTTTAAGTTTGGTTTCGCCATTTCATTACCAATATTGCTTGTTGCAGCGCTTAACTTT
GTATTTGTTCCATTTTCGATACGGTATTTAATAAAGCCTTTTTTTGCACTTCTTATCGCACTTAGTGCAATCGTTAGTTACACAATGATG
AAGTATAGAGTCTTGTTTGATCAAAACATGATTCAGAATATTTTTGAAACCAATCAAAATGAGGCGTTAGCATATTTAAGCTTACCAATT
ATAGGATGGGTTACTATTGCTGGTTTTATCCCTGCCATTTTACTTTTCTTTGTTGAAATTGAATATGAGGAAAAATGGTTCAAAGGGATT
CTAACTCGTGCCCTATCGATGTTTGCATCACTTATAGTGATTGCGGTTATTGCAGCACTATACTATCAAGATTATGTGTCAGTGGGGCGC
AACAATTCAAACCTCCAGCGTGAGATTGTTCCAGCCAATTTCGTTAATAGTACCGTTAAATACGTTTACAATCGTTATCTTGCTGAACCA
ATCCCATTTACAACTTTAGGTGATGATGCAAAACGGGATACTAATCAAAGTAAGCCCACGTTGATGTTTCTGGTCGTTGGTGAAACCGCT
CGTGGTAAAAATTTCTCGATGAATGGCTATGAGAAAGACACCAATCCATTTACCAGTAAATCTGGTGGCGTGATCTCCTTTAATGATGTT
CGTTCGTGTGGGACTGCAACCGCTGTATCTGTCCCCTGCATGTTTTCCAATATGGGGAGAAAGGAGTTTGATGATAATCTCGCTCGTAAT
AGCGAGGGTTTGTTAGATGTGTTGCAGAAAACGGGGGTCTCCATTTTTTGGAAGGAGAACGATGGCGGCTGCAAAGGCGTCTGCGACCGA
GTTCCTAACATCGAGATCAAACCGAAGGATTACCCAAAGTTCTGCGATAAAAACACATGCTATGACGAGGTTGTCCTTCAAGAGCTCGAC
AGTGAAATTGCTCAAATGAAAGGGGATAAGCTGGTTGGCTTCCACCTGATAGGTAGCCATGGCCCAACCTACTACAAACGCTACCCTGAT
GCTCATCGTCAGTTCACTCCTGACTGTCCACGCAGTGATATTGAAAACTGCACAGATGAAGAGCTCACCAACACCTATGACAACACCATC
CGCTACACCGATTTCGTGATTGCAGAGATGATTGCCAAGTTGAAAACCTACGAAGATAAGTACAACACCGCGTTGCTCTACGTCTCCGAT
CATGGTGAATCACTGGGAGCATTAGGGCTTTACCTGCACGGTACACCGTACAAGTTTGCACCGGATGATCAGACCCGTGTTCCTATGCAG
GTGTGGATGTCACCTGGTTTCATCAAAGAAAAAGGCATGAATATGGAATGTTTGCAGAAAAATGCCGCAGCCAATCGCTATTCTCATGAC
AACATATTTTCTTCTGTCCTGGGAATATGGGATGTGAAGACGGCTATCTACGAACAAGAATTAGATATCTTTAAGCAATGTCGGAATAAT
TGA