MCR-3.12

Accession ARO:3004693
DefinitionA plasmid-mediated phosphoethanolamine transferase and MCR-3 variant conferring resistance to colistin antibiotics.
AMR Gene FamilyMCR phosphoethanolamine transferase
Drug Classpeptide antibiotic
Resistance Mechanismantibiotic target alteration
Classification15 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ evolutionary_variant_of MCR-3.1
+ MCR phosphoethanolamine transferase [AMR Gene Family]
Publications

Kieffer N, et al. 2018. Antimicrob. Agents Chemother. 62(7): Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil. (PMID 29712655)

Partridge SR, et al. 2018. J. Antimicrob. Chemother. 73(10):2625-2630 Proposal for assignment of allele numbers for mobile colistin resistance (mcr) genes. (PMID 30053115)

Resistomes

Prevalence of MCR-3.12 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
No prevalence data


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 1000


>gb|AVZ47168.1|+|MCR-3.12 [Escherichia coli]
MPSLIKIKIVPLIFFLALYFAFMLNWRGVLHFYEILYKLEDFKFGFAISLPILLVAALNFAFVPFSIRYLVKPFFALLIALSAIVSYTMM
KYRVLFDQNMIQNIFETNQNEALAYLSLPIIGWVTIAGFIPAILLFFVEIEYEEKWFKGILTRVLSMFASLIVIAVIAALYYQDYVSVGR
NNSNLQREIVPANFVNSTVKYVYNRYLAEPIPFTTLGDDAKRDTNKSKPTLMFLVVGETARGKNFSMNGYEKDTNPFTSKSGGVISFNDV
RSCGTATAVSVPCMFSNMGRKEFDDNLARNSEGLLDVLQKTGVSIFWKENDGGCKGVCDRVPNIEVKPKDYPKFCDKNTCYDEVVLQDLD
SEIAQMKGDKLVGFHLIGSHGPTYYKRYPDAHRQFTPDCPRSDIENCTDEELTNTYDNTIRYTDFVIAEMIAKLKTYEDKYNTALLYVSD
HGESLGAMGLYLHGTPYKFAPDDQTRVPMQVWMSPGFTKEKGMNMECLQQKAADTRYSHDNIFSSVLGIWDVKTSVYEKGLDIFSQCRNV
Q


>gb|MG564491.1|+|1-1626|MCR-3.12 [Escherichia coli]
ATGCCTTCCCTTATAAAAATAAAAATTGTGCCGCTCATATTTTTTTTGGCACTGTATTTTGCATTTATGCTGAACTGGCGTGGAGTTCTC
CATTTTTACGAAATTCTTTATAAATTAGAAGATTTTAAATTTGGTTTCGCCATTTCATTACCAATATTGCTTGTTGCAGCGCTTAATTTT
GCATTTGTTCCATTTTCGATACGGTATTTAGTAAAGCCTTTTTTTGCACTTCTTATCGCACTTAGCGCAATCGTTAGTTACACAATGATG
AAGTATAGAGTCTTGTTTGATCAAAACATGATTCAGAATATTTTTGAAACCAATCAAAATGAGGCATTAGCATATTTAAGTTTGCCAATT
ATAGGATGGGTTACTATTGCTGGATTTATCCCTGCCATTTTACTTTTCTTTGTTGAAATTGAATATGAGGAAAAATGGTTTAAAGGGATT
CTAACTCGCGTCCTATCGATGTTTGCATCCCTTATAGTGATTGCGGTTATTGCAGCACTATACTATCAAGATTATGTTTCAGTGGGGCGC
AACAATTCAAACCTCCAGCGTGAAATTGTTCCGGCCAATTTTGTTAATAGTACCGTTAAATATGTTTATAATCGTTATCTTGCAGAACCA
ATCCCATTTACTACTTTAGGTGATGATGCAAAACGGGATACTAATAAAAGTAAGCCCACGTTGATGTTCCTGGTCGTTGGTGAAACTGCT
CGTGGTAAAAATTTCTCGATGAATGGCTACGAGAAAGATACCAACCCATTTACCAGTAAATCTGGTGGTGTGATCTCCTTTAATGATGTT
CGTTCGTGTGGGACAGCAACCGCTGTATCTGTCCCCTGCATGTTCTCCAATATGGGGAGAAAGGAGTTTGATGATAATCTCGCTCGTAAT
AGCGAGGGTTTGTTAGATGTGTTGCAGAAAACGGGGGTCTCCATTTTTTGGAAGGAGAACGATGGCGGCTGCAAAGGCGTCTGCGACCGA
GTACCTAACATCGAGGTCAAACCGAAGGATTACCCAAAGTTCTGCGATAAAAATACATGCTATGACGAGGTTGTCCTTCAAGACCTCGAT
AGTGAAATTGCTCAAATGAAAGGGGATAAGCTGGTTGGCTTCCACCTGATAGGTAGCCATGGCCCAACCTACTACAAACGCTACCCTGAT
GCTCATCGTCAGTTCACCCCTGATTGTCCACGCAGTGATATCGAAAACTGCACGGATGAAGAGCTCACCAACACCTATGACAACACCATC
CGCTACACCGATTTCGTGATTGCAGAGATGATTGCCAAGTTGAAAACCTACGAAGATAAGTACAACACCGCATTGCTCTACGTCTCCGAT
CACGGTGAATCACTGGGAGCTATGGGGCTTTACCTGCACGGTACACCGTACAAGTTTGCACCGGATGATCAGACCCGCGTACCTATGCAG
GTGTGGATGTCACCTGGATTTACCAAAGAGAAAGGCATGAATATGGAATGTTTGCAGCAGAAAGCCGCTGATACTCGTTACTCACACGAT
AATATTTTCTCATCTGTATTGGGTATCTGGGACGTCAAAACATCAGTTTACGAAAAGGGTCTAGATATTTTCAGTCAATGTCGTAATGTT
CAATAA