| Accession | ARO:3004695 |
| CARD Short Name | MCR-4.3 |
| Definition | A plasmid-mediated MCR-4 variant and colistin resistance gene from clinical Enterobacteriaceae. |
| AMR Gene Family | MCR phosphoethanolamine transferase |
| Drug Class | peptide antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Resistomes with Perfect Matches | Acinetobacter baumanniip, Acinetobacter nosocomialiswgs, Enterobacter cloacaep+wgs, Enterobacter kobeip+wgs, Leclercia adecarboxylatap, Providencia rettgeriwgs |
| Resistomes with Sequence Variants | Acinetobacter baumanniip, Acinetobacter nosocomialisp+wgs, Enterobacter cloacaep+wgs, Enterobacter kobeip+wgs, Leclercia adecarboxylatap, Providencia rettgeriwgs |
| Classification | 14 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + peptide antibiotic [Drug Class] + mechanism of antibiotic resistance + lipopeptide antibiotic + antibiotic target alteration [Resistance Mechanism] + polymyxin antibiotic + determinant of antibiotic resistance + charge alteration conferring antibiotic resistance + colistin + gene altering cell wall charge + phosphoethanolamine transferase conferring colistin resistance + colistin A [Antibiotic] + colistin B [Antibiotic] |
| Parent Term(s) | 4 ontology terms | Show + MCR phosphoethanolamine transferase [AMR Gene Family] + evolutionary_variant_of MCR-4.1 + confers_resistance_to_antibiotic colistin A [Antibiotic] + confers_resistance_to_antibiotic colistin B [Antibiotic] |
| Publications | Partridge SR, et al. 2018. J. Antimicrob. Chemother. 73(10):2625-2630 Proposal for assignment of allele numbers for mobile colistin resistance (mcr) genes. (PMID 30053115) Teo JWP, et al. 2018. J. Clin. Microbiol. 56(3): mcr-3 and mcr-4 Variants in Carbapenemase-Producing Clinical Enterobacteriaceae Do Not Confer Phenotypic Polymyxin Resistance. (PMID 29237785) |
Prevalence of MCR-4.3 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 0% | 0.26% | 0% | 0% |
| Acinetobacter nosocomialis | 0% | 1.64% | 1.72% | 0% |
| Enterobacter cloacae | 0% | 0.56% | 0.64% | 0% |
| Enterobacter kobei | 0% | 0.69% | 0.87% | 0% |
| Leclercia adecarboxylata | 0% | 4.76% | 0% | 0% |
| Providencia rettgeri | 0% | 0% | 0.64% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 1000