| Accession | ARO:3000318 |
| Synonym(s) | macrolide 2'-phosphotransferase II |
| CARD Short Name | mphB |
| Definition | The mphB gene encodes for MPH(2')-II. This enzymes phosphorylates 14-membered and 16-membered macrolides. It phosphorylates macrolides in GTP- dependent manner at 2'-OH hydroxyl of desosamine sugar of macrolides. |
| AMR Gene Family | macrolide phosphotransferase (MPH) |
| Drug Class | macrolide antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Enterobacter roggenkampiiwgs, Escherichia colip+wgs, Escherichia fergusoniiwgs, Klebsiella pneumoniaewgs, Salmonella entericap+wgs, Streptococcus uberiswgs |
| Resistomes with Sequence Variants | Enterobacter hormaecheiwgs, Enterobacter roggenkampiiwgs, Escherichia colip+wgs, Escherichia fergusoniiwgs, Klebsiella pneumoniaewgs, Salmonella entericap+wgs, Streptococcus uberiswgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + antibiotic molecule + macrolide inactivation enzyme + phosphorylation of antibiotic conferring resistance + macrolide antibiotic [Drug Class] |
| Parent Term(s) | 10 ontology terms | Show + confers_resistance_to_antibiotic erythromycin [Antibiotic] + confers_resistance_to_antibiotic roxithromycin [Antibiotic] + confers_resistance_to_antibiotic telithromycin [Antibiotic] + confers_resistance_to_antibiotic clarithromycin [Antibiotic] + confers_resistance_to_antibiotic tylosin [Antibiotic] + confers_resistance_to_antibiotic spiramycin [Antibiotic] + confers_resistance_to_antibiotic azithromycin [Antibiotic] + confers_resistance_to_antibiotic dirithromycin [Antibiotic] + macrolide phosphotransferase (MPH) [AMR Gene Family] + confers_resistance_to_antibiotic oleandomycin [Antibiotic] |
| Publications | Noguchi N, et al. 1996. FEMS Microbiol Lett 144(2-3): 197-202. Cloning and nucleotide sequence of the mphB gene for macrolide 2'-phosphotransferase II in Escherichia coli. (PMID 8900063) Kono M, et al. 1992. FEMS Microbiol Lett 76(1-2): 89-94. Purification and characterization of macrolide 2'-phosphotransferase type II from a strain of Escherichia coli highly resistant to macrolide antibiotics. (PMID 1330822) Noguchi N, et al. 1998. FEMS Microbiol Lett 159(2): 337-342. Expression of the mphB gene for macrolide 2'-phosphotransferase II from Escherichia coli in Staphylococcus aureus. (PMID 9503630) Chesneau O, et al. 2007. FEMS Microbiol Lett 269(2): 317-322. Resistance phenotypes conferred by macrolide phosphotransferases. (PMID 17302923) Pawlowski AC, et al. 2018. Nat Commun 9(1):112 The evolution of substrate discrimination in macrolide antibiotic resistance enzymes. (PMID 29317655) |
Prevalence of mphB among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Enterobacter hormaechei | 0% | 0% | 0.04% | 0% |
| Enterobacter roggenkampii | 0% | 0% | 0.36% | 0% |
| Escherichia coli | 0% | 0.04% | 0.47% | 0% |
| Escherichia fergusonii | 0% | 0% | 1.09% | 0% |
| Klebsiella pneumoniae | 0% | 0% | 0.03% | 0% |
| Salmonella enterica | 0% | 0.11% | 0.09% | 0% |
| Streptococcus uberis | 0% | 0% | 1.79% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 600