| Accession | ARO:3000860 |
| CARD Short Name | rmtB |
| Definition | RmtB is a 16S rRNA methyltransferase that targets mature or nearly mature 30S subunits. It transfers a methyl group from S-adenosyl-L-methionine to N7-G1405 of the 16S rRNA, an aminoglycoside binding site. |
| AMR Gene Family | 16S rRNA methyltransferase (G1405) |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Resistomes with Perfect Matches | Acinetobacter baumanniiwgs, Aeromonas caviaeg, Aeromonas hydrophilawgs, Citrobacter portucalensisp, Enterobacter asburiaewgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheip+wgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniip+wgs, Klebsiella aerogeneswgs, Klebsiella pneumoniaeg+p+wgs, Morganella morganiig+wgs, Proteus mirabilisg+p+wgs+gi, Providencia rettgerig, Providencia stuartiiwgs, Pseudomonas aeruginosag+p+wgs, Salmonella entericag+p+wgs+gi, Shigella flexneriwgs |
| Resistomes with Sequence Variants | Acinetobacter baumanniiwgs, Aeromonas caviaeg, Aeromonas hydrophilawgs, Burkholderia cenocepaciawgs, Citrobacter portucalensisp, Enterobacter asburiaewgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheig+p+wgs+gi, Escherichia colig+p+wgs+gi, Escherichia fergusoniip+wgs, Klebsiella aerogeneswgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaewgs, Morganella morganiig+wgs, Proteus mirabilisg+p+wgs+gi, Providencia rettgerig+wgs, Providencia stuartiiwgs, Pseudomonas aeruginosag+p+wgs+gi, Salmonella entericag+p+wgs+gi, Shigella flexneriwgs |
| Classification | 12 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + ribosomal alteration conferring antibiotic resistance + antibiotic target modifying enzyme + antibiotic molecule + antibiotic mixture + aminoglycoside antibiotic [Drug Class] + rRNA methyltransferase conferring antibiotic resistance + 16S ribosomal RNA methyltransferase + gentamicin [Antibiotic] |
| Parent Term(s) | 12 ontology terms | Show + confers_resistance_to_antibiotic dibekacin [Antibiotic] + confers_resistance_to_antibiotic amikacin [Antibiotic] + confers_resistance_to_antibiotic gentamicin C [Antibiotic] + confers_resistance_to_antibiotic sisomicin [Antibiotic] + confers_resistance_to_antibiotic netilmicin [Antibiotic] + confers_resistance_to_antibiotic kanamycin A [Antibiotic] + confers_resistance_to_antibiotic tobramycin [Antibiotic] + confers_resistance_to_antibiotic isepamicin [Antibiotic] + confers_resistance_to_antibiotic G418 [Antibiotic] + confers_resistance_to_antibiotic arbekacin [Antibiotic] + confers_resistance_to_antibiotic gentamicin B [Antibiotic] + 16S rRNA methyltransferase (G1405) [AMR Gene Family] |
| Publications | Schmitt E, et al. 2009. J Mol Biol 388(3): 570-582. Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases. (PMID 19303884) Zhou Y, et al. 2010. Eur. J. Clin. Microbiol. Infect. Dis. 29(11):1349-53 Distribution of 16S rRNA methylases among different species of Gram-negative bacilli with high-level resistance to aminoglycosides. (PMID 20614151) |
Prevalence of rmtB among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 0% | 0% | 0.03% | 0% |
| Aeromonas caviae | 2.27% | 0% | 0% | 0% |
| Aeromonas hydrophila | 0% | 0% | 0.81% | 0% |
| Burkholderia cenocepacia | 0% | 0% | 1.75% | 0% |
| Citrobacter portucalensis | 0% | 1.47% | 0% | 0% |
| Enterobacter asburiae | 0% | 0% | 0.4% | 0% |
| Enterobacter cloacae | 0% | 1.12% | 1.6% | 0% |
| Enterobacter hormaechei | 0.36% | 0.19% | 0.6% | 3.33% |
| Escherichia coli | 0.14% | 0.63% | 1.29% | 0.13% |
| Escherichia fergusonii | 0% | 0.36% | 1.09% | 0% |
| Klebsiella aerogenes | 0% | 0% | 0.28% | 0% |
| Klebsiella pneumoniae | 0.18% | 2.99% | 4.73% | 0% |
| Klebsiella quasipneumoniae | 0% | 0% | 0.13% | 0% |
| Morganella morganii | 1.92% | 0% | 1.84% | 0% |
| Proteus mirabilis | 3.67% | 2.5% | 0.83% | 3.7% |
| Providencia rettgeri | 2.94% | 0% | 0.64% | 0% |
| Providencia stuartii | 0% | 0% | 2.27% | 0% |
| Pseudomonas aeruginosa | 0.77% | 0.29% | 0.47% | 1.39% |
| Salmonella enterica | 0.38% | 0.44% | 0.1% | 0.66% |
| Shigella flexneri | 0% | 0% | 0.16% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 450
