| Accession | ARO:3004058 |
| CARD Short Name | Saur_23S_LZD |
| Definition | Point mutations in the 23S rRNA subunit of the large ribosomal bacterial subunit in Staphylococcus aureus, which confer resistance to linezolid by disrupting antibiotic target binding. |
| AMR Gene Family | 23S rRNA with mutation conferring resistance to linezolid antibiotics |
| Drug Class | oxazolidinone antibiotic, pleuromutilin antibiotic, phenicol antibiotic, glycopeptide antibiotic, streptogramin antibiotic, lincosamide antibiotic, macrolide antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Resistomes with Sequence Variants | Staphylococcus aureusg+wgs, Staphylococcus capitisg+wgs, Staphylococcus epidermidisg+wgs+gi, Staphylococcus haemolyticusg |
| Classification | 17 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + mutation conferring antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic resistant gene variant or mutant + rRNA with mutation conferring antibiotic resistance + oxazolidinone antibiotic [Drug Class] + pleuromutilin antibiotic [Drug Class] + phenicol antibiotic [Drug Class] + 23S rRNA with mutation conferring antibiotic resistance + glycopeptide antibiotic [Drug Class] + linezolid [Antibiotic] + streptogramin antibiotic [Drug Class] + lincosamide antibiotic [Drug Class] + macrolide antibiotic [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show + confers_resistance_to_antibiotic linezolid [Antibiotic] + 23S rRNA with mutation conferring resistance to linezolid antibiotics [AMR Gene Family] |
| Publications | Musumeci R, et al. 2016. New Microbiol. 39(4):269-273 Resistance to linezolid in Staphylococcus spp. clinical isolates associated with ribosomal binding site modifications: novel mutation in domain V of 23S rRNA. (PMID 27727405) |
Prevalence of Staphylococcus aureus 23S rRNA with mutation conferring resistance to linezolid among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Staphylococcus aureus | 0.26% | 0% | 0.02% | 0% |
| Staphylococcus capitis | 20% | 0% | 1.27% | 0% |
| Staphylococcus epidermidis | 21.29% | 0% | 0.25% | 2.86% |
| Staphylococcus haemolyticus | 6.9% | 0% | 0% | 0% |
Model Type: rRNA gene variant model
Model Definition: Ribosomal RNA (rRNA) Gene Variant Models (RVM) are similar to Protein Variant Models (PVM), i.e. detect sequences based on their similarity to a curated reference sequence and secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles, except RVMs are designed to detect AMR acquired via mutation of genes encoding ribosomal RNAs (rRNA). RVMs include a rRNA reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTN bit-score above the curated BLASTN cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTN bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastN): 5000
Legend:
Published Variants:
| PMID: 27727405 | C2579T G2604T |