mdeA

Accession ARO:3007011
CARD Short NamemdeA
DefinitionmdeA is a multidrug efflux pump that confers resistance in varying degrees to several unrelated antibiotics, including kanamycin, tetracycline, ampicillin, oxacillin, ciprofloxacin, nalidixic acid, acriflavine, and ethidium bromide.
AMR Gene Familymajor facilitator superfamily (MFS) antibiotic efflux pump
Drug Classdisinfecting agents and antiseptics, tetracycline antibiotic, fluoroquinolone antibiotic, penam, aminoglycoside antibiotic
Resistance Mechanismantibiotic efflux
Efflux Componentefflux pump complex or subunit conferring antibiotic resistance
Resistomes with Perfect MatchesStreptococcus mutansg+wgs
Resistomes with Sequence VariantsBacillus cereusg+p+wgs, Bacillus subtiliswgs, Bacillus thuringiensiswgs, Bifidobacterium longumwgs, Clostridium sporogeneswgs, Coxiella burnetiig+wgs, Enterococcus faeciumwgs, Klebsiella pneumoniaewgs, Lactobacillus crispatusg+wgs, Lactobacillus gasserip+wgs, Parascardovia denticolensg+wgs, Pseudomonas aeruginosawgs, Staphylococcus arlettaewgs, Staphylococcus aureusp+wgs, Staphylococcus capitisg+wgs, Staphylococcus epidermidisp+wgs, Staphylococcus equorumwgs, Staphylococcus haemolyticuswgs, Staphylococcus hominisg+wgs, Staphylococcus pasteurig+p+wgs, Staphylococcus warnerig+wgs, Streptococcus gallolyticuswgs, Streptococcus mutansg+wgs, Streptococcus pneumoniaewgs, Streptococcus sobrinusg+wgs, Vibrio choleraewgs
Classification12 ontology terms | Show
Parent Term(s)8 ontology terms | Show
+ major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family]
+ confers_resistance_to_antibiotic kanamycin A [Antibiotic]
+ confers_resistance_to_antibiotic tetracycline [Antibiotic]
+ confers_resistance_to_antibiotic ampicillin [Antibiotic]
+ confers_resistance_to_antibiotic oxacillin [Antibiotic]
+ confers_resistance_to_antibiotic ciprofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic nalidixic acid [Antibiotic]
+ confers_resistance_to_antibiotic acriflavine [Antibiotic]
Publications

Kim DK, et al. 2013. J Microbiol Biotechnol 23(3):430-5 Gene cloning and characterization of MdeA, a novel multidrug efflux pump in Streptococcus mutans. (PMID 23462018)

Resistomes

Prevalence of mdeA among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Bacillus cereus20.43%0.61%9.57%0%
Bacillus subtilis0%0%0.29%0%
Bacillus thuringiensis0%0%0.52%0%
Bifidobacterium longum0%0%0.21%0%
Clostridium sporogenes0%0%0.85%0%
Coxiella burnetii65%0%35.51%0%
Enterococcus faecium0%0%0.07%0%
Escherichia coli0%0%0%0%
Klebsiella pneumoniae0%0%0.01%0%
Lactobacillus crispatus4%0%0.27%0%
Lactobacillus gasseri0%5%5.77%0%
Parascardovia denticolens100%0%100%0%
Pseudomonas aeruginosa0%0%0.01%0%
Staphylococcus arlettae0%0%7.5%0%
Staphylococcus aureus0%0.04%0.1%0%
Staphylococcus capitis50%0%45.57%0%
Staphylococcus epidermidis0%3.46%13.47%0%
Staphylococcus equorum0%0%1.79%0%
Staphylococcus haemolyticus0%0%3.3%0%
Staphylococcus hominis6.25%0%0.49%0%
Staphylococcus pasteuri12.5%29.41%23.08%0%
Staphylococcus warneri8.33%0%23.77%0%
Streptococcus gallolyticus0%0%4.55%0%
Streptococcus mutans100%0%93.75%0%
Streptococcus pneumoniae0%0%0.01%0%
Streptococcus sobrinus100%0%97.96%0%
Vibrio cholerae0%0%0.13%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 200


>gb|WP_002289367.1|-|mdeA [Streptococcus mutans]
MTKHLQDFLITVIAGMGMLLSTLDTGIINVALSFLRGQFQTTTNIASFTITGYTLALAISILPLGVLSDKFGKLKISYLGFVLFGLSSLF
CGFANSIGWLITGRIIQGMGAAALQATSAALITTLVSEDRKNSAISILGVMIGLGPVLGPSLGGILLSLNLWRFIFWINVPFALIVIICN
HYLLTKVSEQTSEWTLDSLGTVLNASMLVCLLLGLSLLSKPHLLGISLILIIISFLIGIWFYRLECSKESALVNVKGLSENPRVLSYLFQ
TVAFGFASAMIFLLPPFIFEQLYHLGVGQTGFLVLGAPAGLVLFSRISGKINDGSKNKSFSHIGLGIIILSLIFLLLLTPSWPYLFLTLG
LFIYGIGGGYFQPANMASIMLASPMKMQGSTGALQRMVQNIAISAGSAIGSTCLNLWSNNLLLATRIGWGITLVLVILSLKELTPFKMTN
KTE


>gb|NZ_CP044221.1|-|1355943-1357304|mdeA [Streptococcus mutans]
ATGACGAAACATTTACAAGATTTTTTAATAACAGTTATCGCAGGTATGGGAATGTTATTATCCACCCTTGATACAGGTATTATCAATGTT
GCATTATCCTTTTTGAGGGGACAATTTCAAACCACAACTAACATTGCCTCTTTTACAATAACTGGATATACTTTAGCATTAGCTATCTCT
ATCTTACCTTTAGGAGTATTAAGCGATAAATTTGGAAAGTTAAAGATTTCCTACTTAGGATTTGTACTTTTTGGACTGAGCTCCTTATTT
TGTGGATTTGCTAATAGTATTGGCTGGCTCATTACTGGCCGGATTATTCAAGGAATGGGAGCGGCGGCTTTGCAAGCTACTTCAGCAGCT
CTGATTACCACTTTAGTTTCCGAAGACCGCAAAAACAGTGCTATTAGTATTTTAGGAGTTATGATTGGCTTAGGACCTGTCCTCGGTCCT
TCCTTAGGAGGCATACTACTTTCTTTAAATCTCTGGCGCTTTATTTTCTGGATTAATGTTCCCTTTGCTCTTATTGTCATCATTTGTAAT
CACTATCTCCTTACTAAAGTCTCCGAACAAACTAGTGAGTGGACATTAGATTCTCTTGGAACAGTTCTCAATGCTTCTATGTTGGTTTGT
CTTCTCTTAGGACTATCCTTACTCAGTAAGCCTCACCTATTAGGTATCAGTCTTATTTTGATCATCATTAGTTTCTTGATCGGGATTTGG
TTTTACCGTCTTGAATGCAGCAAAGAGAGTGCTCTTGTCAATGTCAAGGGGCTGAGCGAGAATCCTCGAGTACTTTCTTATCTTTTTCAA
ACGGTCGCTTTTGGTTTTGCCTCTGCAATGATATTCTTACTTCCACCCTTCATTTTTGAACAATTATATCATTTAGGAGTGGGGCAGACA
GGCTTTCTTGTACTAGGAGCACCTGCAGGCCTGGTTCTTTTTTCAAGAATATCTGGTAAAATCAATGATGGCAGTAAAAATAAATCATTT
AGCCATATTGGTTTGGGTATCATTATTTTATCCTTAATTTTCCTCCTACTTCTCACGCCTAGCTGGCCCTATTTATTCTTAACTTTAGGA
TTATTTATTTATGGTATTGGCGGAGGATACTTTCAGCCTGCTAATATGGCTAGTATTATGCTAGCTAGTCCTATGAAAATGCAAGGTAGC
ACAGGAGCATTGCAACGAATGGTACAAAACATTGCTATTTCAGCTGGTTCAGCCATTGGATCAACTTGCTTAAACCTTTGGTCAAATAAT
CTATTATTGGCTACCCGAATTGGCTGGGGAATTACACTCGTTCTAGTTATTCTCAGTTTAAAAGAACTAACACCATTTAAAATGACTAAT
AAAACAGAATAA