| Accession | ARO:3007011 |
| CARD Short Name | mdeA |
| Definition | mdeA is a multidrug efflux pump that confers resistance in varying degrees to several unrelated antibiotics, including kanamycin, tetracycline, ampicillin, oxacillin, ciprofloxacin, nalidixic acid, acriflavine, and ethidium bromide. |
| AMR Gene Family | major facilitator superfamily (MFS) antibiotic efflux pump |
| Drug Class | disinfecting agents and antiseptics, tetracycline antibiotic, penam, aminoglycoside antibiotic, fluoroquinolone antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Perfect Matches | Streptococcus mutansg+wgs |
| Resistomes with Sequence Variants | Bacillus cereusg+p+wgs, Bacillus subtiliswgs, Bacillus thuringiensiswgs, Bifidobacterium longumwgs, Clostridium sporogeneswgs, Coxiella burnetiig+wgs, Enterococcus faeciumwgs, Klebsiella pneumoniaewgs, Lactobacillus crispatusg+wgs, Lactobacillus gasserip+wgs, Parascardovia denticolensg+wgs, Pseudomonas aeruginosawgs, Staphylococcus arlettaewgs, Staphylococcus aureusp+wgs, Staphylococcus capitisg+wgs, Staphylococcus epidermidisp+wgs, Staphylococcus equorumwgs, Staphylococcus haemolyticuswgs, Staphylococcus hominisg+wgs, Staphylococcus pasteurig+p+wgs, Staphylococcus warnerig+wgs, Streptococcus gallolyticuswgs, Streptococcus mutansg+wgs, Streptococcus pneumoniaewgs, Streptococcus sobrinusg+wgs, Vibrio choleraewgs |
| Classification | 12 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + mechanism of antibiotic resistance + beta-lactam antibiotic + determinant of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + disinfecting agents and antiseptics [Drug Class] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + tetracycline antibiotic [Drug Class] + penam [Drug Class] + aminoglycoside antibiotic [Drug Class] + fluoroquinolone antibiotic [Drug Class] |
| Parent Term(s) | 8 ontology terms | Show + confers_resistance_to_antibiotic ciprofloxacin [Antibiotic] + confers_resistance_to_antibiotic acriflavine [Antibiotic] + confers_resistance_to_antibiotic kanamycin A [Antibiotic] + confers_resistance_to_antibiotic tetracycline [Antibiotic] + confers_resistance_to_antibiotic oxacillin [Antibiotic] + major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family] + confers_resistance_to_antibiotic ampicillin [Antibiotic] + confers_resistance_to_antibiotic nalidixic acid [Antibiotic] |
| Publications | Kim DK, et al. 2013. J Microbiol Biotechnol 23(3):430-5 Gene cloning and characterization of MdeA, a novel multidrug efflux pump in Streptococcus mutans. (PMID 23462018) |
Prevalence of mdeA among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Bacillus cereus | 20.43% | 0.61% | 9.57% | 0% |
| Bacillus subtilis | 0% | 0% | 0.29% | 0% |
| Bacillus thuringiensis | 0% | 0% | 0.52% | 0% |
| Bifidobacterium longum | 0% | 0% | 0.21% | 0% |
| Clostridium sporogenes | 0% | 0% | 0.85% | 0% |
| Coxiella burnetii | 65% | 0% | 35.51% | 0% |
| Enterococcus faecium | 0% | 0% | 0.07% | 0% |
| Escherichia coli | 0% | 0% | 0% | 0% |
| Klebsiella pneumoniae | 0% | 0% | 0.01% | 0% |
| Lactobacillus crispatus | 4% | 0% | 0.27% | 0% |
| Lactobacillus gasseri | 0% | 5% | 5.77% | 0% |
| Parascardovia denticolens | 100% | 0% | 100% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0.01% | 0% |
| Staphylococcus arlettae | 0% | 0% | 7.5% | 0% |
| Staphylococcus aureus | 0% | 0.04% | 0.1% | 0% |
| Staphylococcus capitis | 50% | 0% | 45.57% | 0% |
| Staphylococcus epidermidis | 0% | 3.46% | 13.47% | 0% |
| Staphylococcus equorum | 0% | 0% | 1.79% | 0% |
| Staphylococcus haemolyticus | 0% | 0% | 3.3% | 0% |
| Staphylococcus hominis | 6.25% | 0% | 0.49% | 0% |
| Staphylococcus pasteuri | 12.5% | 29.41% | 23.08% | 0% |
| Staphylococcus warneri | 8.33% | 0% | 23.77% | 0% |
| Streptococcus gallolyticus | 0% | 0% | 4.55% | 0% |
| Streptococcus mutans | 100% | 0% | 93.75% | 0% |
| Streptococcus pneumoniae | 0% | 0% | 0.01% | 0% |
| Streptococcus sobrinus | 100% | 0% | 97.96% | 0% |
| Vibrio cholerae | 0% | 0% | 0.13% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 200