OprN

Accession ARO:3000805
CARD Short NameOprN
DefinitionOprN is the outer membrane channel component of the MexEF-OprN multidrug efflux complex.
AMR Gene Familyresistance-nodulation-cell division (RND) antibiotic efflux pump
Drug Classphenicol antibiotic, diaminopyrimidine antibiotic, fluoroquinolone antibiotic
Resistance Mechanismantibiotic efflux
Efflux Componentefflux pump complex or subunit conferring antibiotic resistance
Resistomes with Perfect MatchesPseudomonas aeruginosag+p+wgs
Resistomes with Sequence VariantsPseudomonas aeruginosag+p+wgs+gi, Pseudomonas fluorescensg
Classification15 ontology terms | Show
Parent Term(s)5 ontology terms | Show
Publications

Kohler T, et al. 1999. J Bacteriol 181(20): 6300-6305. Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa. (PMID 10515918)

Resistomes

Prevalence of OprN among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Pseudomonas aeruginosa97.24%0.29%63.68%1.39%
Pseudomonas fluorescens2.78%0%0%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 800


>gb|AAG05883.1|+|OprN [Pseudomonas aeruginosa PAO1]
MIHAQSIRSGLASALGLFSLLALSACTVGPDYRTPDTAAAKIDATASKPYDRSRFESLWWKQFDDPTLNQLVEQSLSGNRDLRVAFARLR
AARALRDDVANDRFPVVTSRASADIGKGQQPGVTEDRVNSERYDLGLDSAWELDLFGRIRRQLESSDALSEAAEADLQQLQVSLIAELVD
AYGQLRGAQLREKIALSNLENQKESRQLTEQLRDAGVGAELDVLRADARLAATAASVPQLQAEAERARHRIATLLGQRPEELTVDLSPRD
LPAITKALPIGDPGELLRRRPDIRAAERRLAASTADVGVATADLFPRVSLSGFLGFTAGRGSQIGSSAARAWSVGPSISWAAFDLGSVRA
RLRGAKADADAALASYEQQVLLALEESANAFSDYGKRQERLVSLVRQSEASRAAAQQAAIRYREGTTDFLVLLDAEREQLSAEDAQAQAE
VELYRGIVAIYRSLGGGWQPSA


>gb|AE004091.2|+|2813194-2814612|OprN [Pseudomonas aeruginosa PAO1]
ATGATTCACGCGCAGTCGATCCGGAGCGGGCTCGCGTCCGCCCTGGGTCTGTTCAGTCTGCTGGCGCTCAGCGCCTGCACGGTGGGTCCG
GACTACCGGACCCCCGACACCGCGGCGGCGAAGATCGACGCCACGGCGAGCAAGCCCTACGACCGCAGCCGCTTCGAAAGCCTGTGGTGG
AAACAGTTCGACGATCCGACCCTGAACCAGTTGGTCGAACAGTCGCTGAGCGGCAACCGCGACCTGCGCGTGGCCTTCGCCCGCCTGCGC
GCCGCCCGCGCCCTGCGCGACGACGTGGCCAACGATCGCTTCCCGGTGGTCACCAGCCGCGCCAGCGCCGACATCGGCAAGGGCCAGCAA
CCGGGAGTGACCGAGGACCGGGTCAACAGCGAGCGCTACGACCTTGGCCTGGATAGCGCCTGGGAGCTTGACCTGTTCGGGCGCATCCGC
CGTCAGCTGGAGTCCAGCGACGCCCTCAGCGAAGCGGCCGAGGCCGACCTGCAGCAACTGCAGGTCAGCCTGATCGCCGAGCTGGTGGAC
GCCTACGGCCAACTGCGCGGCGCGCAACTGCGCGAGAAGATTGCCCTGAGCAACCTGGAGAACCAGAAGGAGTCGCGCCAGCTCACCGAG
CAACTGCGCGACGCCGGGGTCGGTGCCGAACTCGACGTACTGCGCGCCGATGCGCGCCTGGCGGCCACCGCCGCCAGCGTGCCGCAACTG
CAGGCGGAAGCCGAGCGCGCCAGGCACCGTATCGCCACCCTCCTCGGCCAACGGCCGGAAGAGTTGACAGTGGACCTTTCGCCGCGCGAC
CTGCCGGCGATCACCAAGGCCCTGCCGATCGGCGATCCCGGCGAACTGCTGCGCCGCCGGCCGGACATCCGCGCCGCCGAACGGCGCCTG
GCCGCCAGCACCGCCGACGTCGGCGTGGCCACCGCCGACCTGTTCCCGCGGGTCAGCCTCAGCGGCTTCCTCGGCTTCACCGCCGGGCGG
GGCTCGCAGATCGGCTCAAGCGCCGCCCGCGCCTGGAGCGTCGGCCCGAGCATCAGTTGGGCCGCCTTCGACCTCGGCAGCGTGCGTGCC
CGCCTGCGCGGCGCCAAGGCCGACGCCGACGCCGCGCTGGCCAGCTACGAACAGCAGGTGCTGCTGGCCCTGGAAGAATCGGCGAATGCC
TTCAGCGACTATGGCAAGCGCCAGGAGCGCCTGGTCTCGCTGGTCCGCCAGTCGGAAGCCAGCCGCGCCGCCGCGCAACAGGCGGCGATC
CGCTACCGCGAAGGCACCACCGATTTCCTGGTGCTGCTGGACGCCGAACGCGAGCAACTCTCCGCCGAAGATGCCCAGGCCCAGGCCGAG
GTCGAGCTGTACCGCGGCATCGTGGCGATCTACCGCTCCCTCGGCGGTGGCTGGCAACCCAGCGCCTGA