| Accession | ARO:3001866 |
| Synonym(s) | CTX-M-133 |
| CARD Short Name | CTX-M-3 |
| Definition | CTX-M-3 is a beta-lactamase found in Citrobacter freundii. |
| AMR Gene Family | CTX-M beta-lactamase |
| Drug Class | penam, cephamycin, cephalosporin |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Aeromonas caviaeg+p+wgs, Aeromonas hydrophilag, Aeromonas veroniig+p, Citrobacter amalonaticuswgs, Citrobacter freundiiwgs, Citrobacter portucalensiswgs, Enterobacter asburiaewgs, Enterobacter chengduensiswgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheip+wgs, Enterobacter kobeig+p+wgs, Enterobacter roggenkampiiwgs, Escherichia colig+p+wgs, Escherichia fergusoniip+wgs, Klebsiella aerogeneswgs, Klebsiella michiganensisp+wgs, Klebsiella oxytocawgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaep+wgs, Morganella morganiig+wgs, Proteus mirabilisg+wgs+gi, Providencia rettgerig+wgs, Pseudomonas aeruginosawgs, Raoultella planticolap, Salmonella entericawgs, Serratia marcescensp+wgs, Shigella flexnerip+wgs, Shigella sonneip+wgs |
| Resistomes with Sequence Variants | Aeromonas caviaeg+p+wgs, Aeromonas hydrophilag, Aeromonas veroniig+p, Citrobacter amalonaticuswgs, Citrobacter freundiiwgs, Citrobacter portucalensiswgs, Enterobacter asburiaewgs, Enterobacter chengduensiswgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheip+wgs, Enterobacter kobeig+p+wgs, Enterobacter roggenkampiiwgs, Escherichia colig+p+wgs, Escherichia fergusoniip+wgs, Klebsiella aerogeneswgs, Klebsiella michiganensisp+wgs, Klebsiella oxytocawgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaep+wgs, Morganella morganiig+wgs, Proteus mirabilisg+wgs+gi, Providencia rettgerig+wgs, Pseudomonas aeruginosawgs, Raoultella planticolap, Salmonella entericawgs, Serratia marcescensp+wgs, Shigella flexnerip+wgs, Shigella sonneip+wgs |
| Classification | 15 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + hydrolysis of antibiotic conferring resistance + beta-lactam antibiotic + cephem + hydrolysis of beta-lactam antibiotic by serine beta-lactamase + beta-lactamase + class A beta-lactamase + penam [Drug Class] + cephamycin [Drug Class] + cephalosporin [Drug Class] |
| Parent Term(s) | 8 ontology terms | Show + confers_resistance_to_antibiotic cefoxitin [Antibiotic] + confers_resistance_to_antibiotic cefazolin [Antibiotic] + confers_resistance_to_antibiotic ceftazidime [Antibiotic] + confers_resistance_to_antibiotic ceftriaxone [Antibiotic] + CTX-M beta-lactamase [AMR Gene Family] + confers_resistance_to_antibiotic ampicillin [Antibiotic] + confers_resistance_to_antibiotic cefixime [Antibiotic] + confers_resistance_to_antibiotic cefalotin [Antibiotic] |
| Sub-Term(s) | 1 ontology terms | Show + vaborbactam [Adjuvant] is_small_molecule_inhibitor |
| Publications | Gniadkowski M, et al. 1998. Antimicrob Agents Chemother 42(4): 827-832. Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaxime-hydrolyzing beta-lactamase that is closely related to the CTX-M-1/MEN-1 enzyme. (PMID 9559791) Tsang KK, et al. 2021. Microb Genom 7(1): Identifying novel β-lactamase substrate activity through in silico prediction of antimicrobial resistance. (PMID 33416461) |
Prevalence of CTX-M-3 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Aeromonas caviae | 4.55% | 1.3% | 0.54% | 0% |
| Aeromonas hydrophila | 4.62% | 0% | 0% | 0% |
| Aeromonas veronii | 3.64% | 1.54% | 0% | 0% |
| Citrobacter amalonaticus | 0% | 0% | 1.82% | 0% |
| Citrobacter freundii | 0% | 0% | 2.32% | 0% |
| Citrobacter portucalensis | 0% | 0% | 1.8% | 0% |
| Enterobacter asburiae | 0% | 0% | 1.98% | 0% |
| Enterobacter chengduensis | 0% | 0% | 4% | 0% |
| Enterobacter cloacae | 0% | 1.12% | 1.6% | 0% |
| Enterobacter hormaechei | 0% | 0.39% | 1.55% | 0% |
| Enterobacter kobei | 4.55% | 1.38% | 3.93% | 0% |
| Enterobacter roggenkampii | 0% | 0% | 0.72% | 0% |
| Escherichia coli | 0.05% | 0.08% | 0.39% | 0% |
| Escherichia fergusonii | 0% | 0.36% | 2.17% | 0% |
| Klebsiella aerogenes | 0% | 0% | 0.56% | 0% |
| Klebsiella michiganensis | 0% | 1.14% | 0.8% | 0% |
| Klebsiella oxytoca | 0% | 0% | 0.84% | 0% |
| Klebsiella pneumoniae | 0.18% | 0.61% | 1.14% | 0% |
| Klebsiella quasipneumoniae | 0% | 0.85% | 2.5% | 0% |
| Morganella morganii | 3.85% | 0% | 2.45% | 0% |
| Proteus mirabilis | 4.59% | 0% | 0.5% | 7.41% |
| Providencia rettgeri | 8.82% | 0% | 1.27% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0.03% | 0% |
| Raoultella planticola | 0% | 2.33% | 0% | 0% |
| Salmonella enterica | 0% | 0% | 0.01% | 0% |
| Serratia marcescens | 0% | 0.65% | 0.13% | 0% |
| Shigella flexneri | 0% | 1.2% | 0.78% | 0% |
| Shigella sonnei | 0% | 4.85% | 0.22% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 500