Accession ARO:3000216
Synonym(s)acrE ECK0456 JW0451
DefinitionProtein subunit of AcrA-AcrB-TolC multidrug efflux complex. AcrB functions as a herterotrimer which forms the inner membrane component and is primarily responsible for substrate recognition and energy transduction by acting as a drug/proton antiporter.
AMR Gene Familyresistance-nodulation-cell division (RND) antibiotic efflux pump
Drug Classtetracycline antibiotic, penam, phenicol antibiotic, glycylcycline, rifamycin antibiotic, cephalosporin, triclosan, fluoroquinolone antibiotic
Resistance Mechanismantibiotic efflux
Efflux Componentefflux pump complex or subunit conferring antibiotic resistance
ResistomesEscherichia colig+wgs, Klebsiella oxytocawgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneiwgs
Classification24 ontology terms | Show
Parent Term(s)2 ontology terms | Show
Publications

Tornroth-Horsefield S, et al. 2007. Structure 15(12): 1663-1673. Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist. (PMID 18073115)

Murakami S, et al. 2002. Nature 419(6907): 587-593. Crystal structure of bacterial multidrug efflux transporter AcrB. (PMID 12374972)

Das D, et al. 2007. J Struct Biol 158(3): 494-502. Crystal structure of the multidrug efflux transporter AcrB at 3.1A resolution reveals the N-terminal region with conserved amino acids. (PMID 17275331)

Pos KM. 2009. Biochim Biophys Acta 1794(5): 782-793. Drug transport mechanism of the AcrB efflux pump. (PMID 19166984)

Eicher T, et al. 2009. Biol Chem 390(8): 693-699. Structural and functional aspects of the multidrug efflux pump AcrB. (PMID 19453279)

Resistomes

Prevalence of acrB among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
Acinetobacter baumannii0%0%0.04%
Citrobacter amalonaticus66.67%0%88.89%
Citrobacter freundii96.55%0%92.25%
Citrobacter koseri0%0%4.17%
Citrobacter youngae50%0%50%
Enterobacter asburiae0%0%1.56%
Enterobacter cloacae0%0%0.23%
Enterobacter hormaechei0%0%2.22%
Enterobacter kobei0%0%1.56%
Enterococcus faecium0%0%0.07%
Escherichia coli13.02%0%86.34%
Klebsiella oxytoca0%0%1.87%
Klebsiella pneumoniae0%0%0%
Morganella morganii0%0%0%
Proteus mirabilis0%0%0%
Pseudomonas aeruginosa0%0%0%
Salmonella enterica85.66%0%57.66%
Serratia marcescens0%0%0.69%
Shigella dysenteriae100%0%100%
Shigella flexneri100%0%99.48%
Shigella sonnei100%0%99.84%
Staphylococcus aureus0%0%0.01%
Vibrio parahaemolyticus0%0%0.25%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 1900


>gb|AAC73564.1|-|acrB [Escherichia coli str. K-12 substr. MG1655]
MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVTISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQI
TLTFESGTDADIAQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAANMKDAISRTSGVGDVQLFGS
QYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQLGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIEL
GGENYDIIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQ
NFRATLIPTIAVPVVLLGTFAVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEGKKGFFGWFNRMFEKSTHHYTDSVGGILRSTGR
YLVLYLIIVVGMAYLFVRLPSSFLPDEDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNTGIAFVS
LKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDFELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNG
LEDTPQFKIDIDQEKAQALGVSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQMVPFSAFSSSRWE
YGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVGYDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFS
VMLVVPLGVIGALLAATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRLRPILMTSLAFILGVMPLV
ISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVRRRFSRKNEDIEHSHTVDHH


>gb|U00096.3|-|481254-484403|acrB [Escherichia coli str. K-12 substr. MG1655]
ATGCCTAATTTCTTTATCGATCGCCCGATTTTTGCGTGGGTGATCGCCATTATCATCATGTTGGCAGGGGGGCTGGCGATCCTCAAACTG
CCGGTGGCGCAATATCCTACGATTGCACCGCCGGCAGTAACGATCTCCGCCTCCTACCCCGGCGCTGATGCGAAAACAGTGCAGGACACG
GTGACACAGGTTATCGAACAGAATATGAACGGTATCGATAACCTGATGTACATGTCCTCTAACAGTGACTCCACGGGTACCGTGCAGATC
ACCCTGACCTTTGAGTCTGGTACTGATGCGGATATCGCGCAGGTTCAGGTGCAGAACAAACTGCAGCTGGCGATGCCGTTGCTGCCGCAA
GAAGTTCAGCAGCAAGGGGTGAGCGTTGAGAAATCATCCAGCAGCTTCCTGATGGTTGTCGGCGTTATCAACACCGATGGCACCATGACG
CAGGAGGATATCTCCGACTACGTGGCGGCGAATATGAAAGATGCCATCAGCCGTACGTCGGGCGTGGGTGATGTTCAGTTGTTCGGTTCA
CAGTACGCGATGCGTATCTGGATGAACCCGAATGAGCTGAACAAATTCCAGCTAACGCCGGTTGATGTCATTACCGCCATCAAAGCGCAG
AACGCCCAGGTTGCGGCGGGTCAGCTCGGTGGTACGCCGCCGGTGAAAGGCCAACAGCTTAACGCCTCTATTATTGCTCAGACGCGTCTG
ACCTCTACTGAAGAGTTCGGCAAAATCCTGCTGAAAGTGAATCAGGATGGTTCCCGCGTGCTGCTGCGTGACGTCGCGAAGATTGAGCTG
GGTGGTGAGAACTACGACATCATCGCAGAGTTTAACGGCCAACCGGCTTCCGGTCTGGGGATCAAGCTGGCGACCGGTGCAAACGCGCTG
GATACCGCTGCGGCAATCCGTGCTGAACTGGCGAAGATGGAACCGTTCTTCCCGTCGGGTCTGAAAATTGTTTACCCATACGACACCACG
CCGTTCGTGAAAATCTCTATTCACGAAGTGGTTAAAACGCTGGTCGAAGCGATCATCCTCGTGTTCCTGGTTATGTATCTGTTCCTGCAG
AACTTCCGCGCGACGTTGATTCCGACCATTGCCGTACCGGTGGTATTGCTCGGGACCTTTGCCGTCCTTGCCGCCTTTGGCTTCTCGATA
AACACGCTAACAATGTTCGGGATGGTGCTCGCCATCGGCCTGTTGGTGGATGACGCCATCGTTGTGGTAGAAAACGTTGAGCGTGTTATG
GCGGAAGAAGGTTTGCCGCCAAAAGAAGCTACCCGTAAGTCGATGGGGCAGATTCAGGGCGCTCTGGTCGGTATCGCGATGGTACTGTCG
GCGGTATTCGTACCGATGGCCTTCTTTGGCGGTTCTACTGGTGCTATCTATCGTCAGTTCTCTATTACCATTGTTTCAGCAATGGCGCTG
TCGGTACTGGTGGCGTTGATCCTGACTCCAGCTCTTTGTGCCACCATGCTGAAACCGATTGCCAAAGGCGATCACGGGGAAGGTAAAAAA
GGCTTCTTCGGCTGGTTTAACCGCATGTTCGAGAAGAGCACGCACCACTACACCGACAGCGTAGGCGGTATTCTGCGCAGTACGGGGCGT
TACCTGGTGCTGTATCTGATCATCGTGGTCGGCATGGCCTATCTGTTCGTGCGTCTGCCAAGCTCCTTCTTGCCAGATGAGGACCAGGGC
GTGTTTATGACCATGGTTCAGCTGCCAGCAGGTGCAACGCAGGAACGTACACAGAAAGTGCTCAATGAGGTAACGCATTACTATCTGACC
AAAGAAAAGAACAACGTTGAGTCGGTGTTCGCCGTTAACGGCTTCGGCTTTGCGGGACGTGGTCAGAATACCGGTATTGCGTTCGTTTCC
TTGAAGGACTGGGCCGATCGTCCGGGCGAAGAAAACAAAGTTGAAGCGATTACCATGCGTGCAACACGCGCTTTCTCGCAAATCAAAGAT
GCGATGGTTTTCGCCTTTAACCTGCCCGCAATCGTGGAACTGGGTACTGCAACCGGCTTTGACTTTGAGCTGATTGACCAGGCTGGCCTT
GGTCACGAAAAACTGACTCAGGCGCGTAACCAGTTGCTTGCAGAAGCAGCGAAGCACCCTGATATGTTGACCAGCGTACGTCCAAACGGT
CTGGAAGATACCCCGCAGTTTAAGATTGATATCGACCAGGAAAAAGCGCAGGCGCTGGGTGTTTCTATCAACGACATTAACACCACTCTG
GGCGCTGCATGGGGCGGCAGCTATGTGAACGACTTTATCGACCGCGGTCGTGTGAAGAAAGTTTATGTCATGTCAGAAGCGAAATACCGT
ATGCTGCCGGATGATATCGGCGACTGGTATGTTCGTGCTGCTGATGGTCAGATGGTGCCATTCTCGGCGTTCTCCTCTTCTCGTTGGGAG
TACGGTTCGCCGCGTCTGGAACGTTACAACGGCCTGCCATCCATGGAAATCTTAGGCCAGGCGGCACCGGGTAAAAGTACCGGTGAAGCA
ATGGAGCTGATGGAACAACTGGCGAGCAAACTGCCTACCGGTGTTGGCTATGACTGGACGGGGATGTCCTATCAGGAACGTCTCTCCGGC
AACCAGGCACCTTCACTGTACGCGATTTCGTTGATTGTCGTGTTCCTGTGTCTGGCGGCGCTGTACGAGAGCTGGTCGATTCCGTTCTCC
GTTATGCTGGTCGTTCCGCTGGGGGTTATCGGTGCGTTGCTGGCTGCCACCTTCCGTGGCCTGACCAATGACGTTTACTTCCAGGTAGGC
CTGCTCACAACCATTGGGTTGTCGGCGAAGAACGCGATCCTTATCGTCGAATTCGCCAAAGACTTGATGGATAAAGAAGGTAAAGGTCTG
ATTGAAGCGACGCTTGATGCGGTGCGGATGCGTTTACGTCCGATCCTGATGACCTCGCTGGCGTTTATCCTCGGCGTTATGCCGCTGGTT
ATCAGTACTGGTGCTGGTTCCGGCGCGCAGAACGCAGTAGGTACCGGTGTAATGGGCGGGATGGTGACCGCAACGGTACTGGCAATCTTC
TTCGTTCCGGTATTCTTTGTGGTGGTTCGCCGCCGCTTTAGCCGCAAGAATGAAGATATCGAGCACAGCCATACTGTCGATCATCATTGA