| Accession | ARO:3003378 |
| CARD Short Name | Ecol_MarR_MULT |
| Definition | MarR is a repressor of the mar operon marRAB, thus regulating the expression of marA, the activator of multidrug efflux pump AcrAB. |
| AMR Gene Family | resistance-nodulation-cell division (RND) antibiotic efflux pump |
| Drug Class | tetracycline antibiotic, disinfecting agents and antiseptics, phenicol antibiotic, rifamycin antibiotic, penam, glycylcycline, cephalosporin, fluoroquinolone antibiotic |
| Resistance Mechanism | antibiotic efflux, antibiotic target alteration |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Efflux Regulator | protein(s) and two-component regulatory system modulating antibiotic efflux |
| Resistomes with Sequence Variants | Citrobacter amalonaticusg+wgs, Citrobacter freundiig+wgs, Citrobacter koserig+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniig+wgs, Citrobacter youngaeg+wgs, Cronobacter condimentig+wgs, Cronobacter dublinensisg+wgs, Cronobacter malonaticusg+wgs, Cronobacter sakazakiig+wgs, Cronobacter turicensiswgs, Cronobacter universalisg+wgs, Enterobacter asburiaeg+wgs, Enterobacter cancerogenusg+wgs, Enterobacter chengduensisg+wgs, Enterobacter cloacaeg+wgs, Enterobacter hormaecheig+wgs, Enterobacter kobeig+wgs, Enterobacter roggenkampiig+wgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs, Escherichia fergusoniig+wgs, Escherichia marmotaeg+wgs, Klebsiella aerogenesg+wgs, Klebsiella huaxiensisg+wgs, Klebsiella michiganensisg+wgs, Klebsiella oxytocag+wgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaeg+wgs, Kosakonia arachidisg+wgs, Leclercia adecarboxylatag+wgs, Raoultella planticolag+wgs, Salmonella bongorig+wgs, Salmonella entericag+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Classification | 30 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + beta-lactam antibiotic + mechanism of antibiotic resistance + cephem + tetracycline antibiotic [Drug Class] + determinant of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + antibiotic target alteration [Resistance Mechanism] + disinfecting agents and antiseptics [Drug Class] + phenicol antibiotic [Drug Class] + mutation conferring antibiotic resistance + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + rifamycin antibiotic [Drug Class] + penam [Drug Class] + glycylcycline [Drug Class] + cephalosporin [Drug Class] + triclosan [Antibiotic] + cefalotin [Antibiotic] + ampicillin [Antibiotic] + chloramphenicol [Antibiotic] + rifampin [Antibiotic] + resistance-nodulation-cell division (RND) antibiotic efflux pump [AMR Gene Family] + tetracycline [Antibiotic] + antibiotic resistant gene variant or mutant + tigecycline [Antibiotic] + fluoroquinolone antibiotic [Drug Class] + protein(s) and two-component regulatory system modulating antibiotic efflux [Efflux Regulator] + AcrAB-TolC + mutant efflux regulatory protein conferring antibiotic resistance |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic ciprofloxacin [Antibiotic] + confers_resistance_to_antibiotic tetracycline [Antibiotic] + marR |
| Publications | Alekshun MN and Levy SB. 1997. Antimicrob Agents Chemother 41(10): 2067-2075. Regulation of chromosomally mediated multiple antibiotic resistance: the mar regulon. (PMID 9333027) Randall LP and Woodward MJ. 2002. Res Vet Sci 72(2): 87-93. The multiple antibiotic resistance (mar) locus and its significance. (PMID 12027588) Oethinger M, et al. 1998. Antimicrob. Agents Chemother. 42(8):2089-94 Overexpression of the marA or soxS regulatory gene in clinical topoisomerase mutants of Escherichia coli. (PMID 9687412) Maneewannakul K, et al. 1996. Antimicrob. Agents Chemother. 40(7):1695-8 Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli. (PMID 8807064) Okusu H, et al. 1996. J Bacteriol 178(1): 306-308. AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants. (PMID 8550435) Alekshun MN, et al. 2000. Mol Microbiol 35(6):1394-404 Mutational analysis of MarR, the negative regulator of marRAB expression in Escherichia coli, suggests the presence of two regions required for DNA binding. (PMID 10760140) Webber MA, et al. 2001. Antimicrob. Agents Chemother. 45(5):1550-2 Absence of mutations in marRAB or soxRS in acrB-overexpressing fluoroquinolone-resistant clinical and veterinary isolates of Escherichia coli. (PMID 11302826) |
Prevalence of Escherichia coli AcrAB-TolC with MarR mutations conferring resistance to ciprofloxacin and tetracycline among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Citrobacter amalonaticus | 100% | 0% | 89.09% | 0% |
| Citrobacter freundii | 100% | 0% | 50.87% | 0% |
| Citrobacter koseri | 100% | 0% | 49.55% | 0% |
| Citrobacter portucalensis | 100% | 0% | 62.16% | 0% |
| Citrobacter werkmanii | 100% | 0% | 61.54% | 0% |
| Citrobacter youngae | 100% | 0% | 100% | 0% |
| Cronobacter condimenti | 100% | 0% | 100% | 0% |
| Cronobacter dublinensis | 100% | 0% | 100% | 0% |
| Cronobacter malonaticus | 100% | 0% | 85.45% | 0% |
| Cronobacter sakazakii | 100% | 0% | 92.15% | 0% |
| Cronobacter turicensis | 0% | 0% | 83.33% | 0% |
| Cronobacter universalis | 100% | 0% | 66.67% | 0% |
| Enterobacter asburiae | 100% | 0% | 70.75% | 0% |
| Enterobacter cancerogenus | 100% | 0% | 100% | 0% |
| Enterobacter chengduensis | 100% | 0% | 84% | 0% |
| Enterobacter cloacae | 98.21% | 0% | 73.16% | 0% |
| Enterobacter hormaechei | 98.56% | 0% | 66.77% | 0% |
| Enterobacter kobei | 100% | 0% | 68.12% | 0% |
| Enterobacter roggenkampii | 100% | 0% | 62.23% | 0% |
| Escherichia albertii | 95.71% | 0% | 60.65% | 0% |
| Escherichia coli | 67.65% | 0.06% | 61.51% | 0% |
| Escherichia fergusonii | 100% | 0% | 51.09% | 0% |
| Escherichia marmotae | 100% | 0% | 70.83% | 0% |
| Klebsiella aerogenes | 100% | 0% | 79.66% | 0% |
| Klebsiella huaxiensis | 100% | 0% | 66.67% | 0% |
| Klebsiella michiganensis | 100% | 0% | 69.15% | 0% |
| Klebsiella oxytoca | 100% | 0% | 74.37% | 0% |
| Klebsiella pneumoniae | 99.11% | 0.01% | 57.19% | 0% |
| Klebsiella quasipneumoniae | 100% | 0% | 73.42% | 0% |
| Kosakonia arachidis | 100% | 0% | 100% | 0% |
| Leclercia adecarboxylata | 100% | 0% | 60.47% | 0% |
| Raoultella planticola | 100% | 0% | 94.87% | 0% |
| Salmonella bongori | 100% | 0% | 94.74% | 0% |
| Salmonella enterica | 95.39% | 0% | 80.78% | 0% |
| Shigella boydii | 93.33% | 0% | 94.44% | 0% |
| Shigella dysenteriae | 100% | 0% | 90% | 0% |
| Shigella flexneri | 100% | 0% | 82.14% | 0% |
| Shigella sonnei | 100% | 0% | 94.01% | 0% |
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| No prevalence data | ||||
Model Type: protein overexpression model
Model Definition: Protein Overexpression Models (POM) are similar to Protein Variant Models (PVM) in that they include a protein reference sequence, a curated BLASTP bitscore cut-off, and mapped resistance variants. Whereas PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, reporting only those with curated mutations conferring AMR, POMs are restricted to regulatory proteins and report both wild-type sequences and/or sequences with mutations leading to overexpression of efflux complexes. The former lead to efflux of antibiotics at basal levels, while the latter can confer clinical resistance. POMs include a protein reference sequence (often from wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Perfect RGI match is 100% identical to the wild-type reference protein sequence along its entire length, a Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value may or may not contain at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off may or may not contain at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 210
Legend:
Published Variants:
| PMID: 9687412 | E31STOP I49S L78M R94S R94H |
| PMID: 8807064 | S3N V96E G103S |
| PMID: 8550435 | R58L |
| PMID: 10760140 | V45E A70T R73C R73S R77C R77L |
| PMID: 11302826 | Y137H |
Model Name: Escherichia coli AcrAB-TolC with MarR mutations conferring resistance to ciprofloxacin and tetracycline
Model Description: The Escherichia coli AcrAB-TolC with MarR mutation (Y137H) conferring resistance to ciprofloxacin and tetracycline.
Escherichia coli marR mutant conferring antibiotic resistanceTolCacrBEscherichia coli acrA