| Accession | ARO:3003378 |
| CARD Short Name | Ecol_MarR_MULT |
| Definition | MarR is a repressor of the mar operon marRAB, thus regulating the expression of marA, the activator of multidrug efflux pump AcrAB. |
| AMR Gene Family | resistance-nodulation-cell division (RND) antibiotic efflux pump |
| Drug Class | tetracycline antibiotic, disinfecting agents and antiseptics, phenicol antibiotic, cephalosporin, penam, rifamycin antibiotic, glycylcycline, fluoroquinolone antibiotic |
| Resistance Mechanism | antibiotic efflux, antibiotic target alteration |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Efflux Regulator | protein(s) and two-component regulatory system modulating antibiotic efflux |
| Resistomes with Sequence Variants | Citrobacter amalonaticusg+wgs, Citrobacter freundiig+wgs, Citrobacter koserig+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniig+wgs, Citrobacter youngaeg+wgs, Cronobacter sakazakiig+wgs, Enterobacter asburiaeg+wgs, Enterobacter chengduensisg+wgs, Enterobacter cloacaeg+wgs, Enterobacter hormaecheig+wgs, Enterobacter kobeig+wgs, Enterobacter roggenkampiig+wgs, Enterococcus faeciumwgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs, Escherichia fergusoniig+wgs, Escherichia marmotaeg+wgs, Klebsiella aerogenesg+wgs, Klebsiella huaxiensisg+wgs, Klebsiella michiganensisg+wgs, Klebsiella oxytocag+wgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaeg+wgs, Kosakonia arachidisg+wgs, Leclercia adecarboxylatag+wgs, Pseudomonas aeruginosawgs, Raoultella planticolag+wgs, Salmonella entericag+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Classification | 30 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + beta-lactam antibiotic + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + tetracycline antibiotic [Drug Class] + cephem + antibiotic target alteration [Resistance Mechanism] + disinfecting agents and antiseptics [Drug Class] + phenicol antibiotic [Drug Class] + cephalosporin [Drug Class] + mutation conferring antibiotic resistance + penam [Drug Class] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + rifamycin antibiotic [Drug Class] + glycylcycline [Drug Class] + antibiotic resistant gene variant or mutant + fluoroquinolone antibiotic [Drug Class] + chloramphenicol [Antibiotic] + ampicillin [Antibiotic] + triclosan [Antibiotic] + rifampin [Antibiotic] + tetracycline [Antibiotic] + resistance-nodulation-cell division (RND) antibiotic efflux pump [AMR Gene Family] + cefalotin [Antibiotic] + tigecycline [Antibiotic] + AcrAB-TolC + protein(s) and two-component regulatory system modulating antibiotic efflux [Efflux Regulator] + mutant efflux regulatory protein conferring antibiotic resistance |
| Parent Term(s) | 1 ontology terms | Show + marR |
| Publications | Alekshun MN and Levy SB. 1997. Antimicrob Agents Chemother 41(10): 2067-2075. Regulation of chromosomally mediated multiple antibiotic resistance: the mar regulon. (PMID 9333027) Randall LP and Woodward MJ. 2002. Res Vet Sci 72(2): 87-93. The multiple antibiotic resistance (mar) locus and its significance. (PMID 12027588) Oethinger M, et al. 1998. Antimicrob. Agents Chemother. 42(8):2089-94 Overexpression of the marA or soxS regulatory gene in clinical topoisomerase mutants of Escherichia coli. (PMID 9687412) Maneewannakul K, et al. 1996. Antimicrob. Agents Chemother. 40(7):1695-8 Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli. (PMID 8807064) Okusu H, et al. 1996. J Bacteriol 178(1): 306-308. AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants. (PMID 8550435) |
Prevalence of Escherichia coli AcrAB-TolC with MarR mutations conferring resistance to ciprofloxacin and tetracycline among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 263 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Citrobacter amalonaticus | 100% | 0% | 87.5% | 0% |
| Citrobacter freundii | 100% | 0% | 60.76% | 0% |
| Citrobacter koseri | 100% | 0% | 46.67% | 0% |
| Citrobacter portucalensis | 100% | 0% | 86% | 0% |
| Citrobacter werkmanii | 100% | 0% | 100% | 0% |
| Citrobacter youngae | 100% | 0% | 100% | 0% |
| Cronobacter sakazakii | 100% | 0% | 92.88% | 0% |
| Enterobacter asburiae | 100% | 0% | 88.19% | 0% |
| Enterobacter chengduensis | 100% | 0% | 90.91% | 0% |
| Enterobacter cloacae | 97.73% | 0% | 81.82% | 0% |
| Enterobacter hormaechei | 98.81% | 0% | 80.53% | 0% |
| Enterobacter kobei | 91.67% | 0% | 86.89% | 0% |
| Enterobacter roggenkampii | 100% | 0% | 73.94% | 0% |
| Enterococcus faecium | 0% | 0% | 0.04% | 0% |
| Escherichia albertii | 95% | 0% | 94.25% | 0% |
| Escherichia coli | 61.38% | 0.07% | 74.4% | 0% |
| Escherichia fergusonii | 100% | 0% | 44.62% | 0% |
| Escherichia marmotae | 100% | 0% | 69.05% | 0% |
| Klebsiella aerogenes | 100% | 0% | 81.45% | 0% |
| Klebsiella huaxiensis | 100% | 0% | 50% | 0% |
| Klebsiella michiganensis | 97.67% | 0% | 75.65% | 0% |
| Klebsiella oxytoca | 100% | 0% | 81.11% | 0% |
| Klebsiella pneumoniae | 98.97% | 0.02% | 71.71% | 0% |
| Klebsiella quasipneumoniae | 100% | 0% | 81.18% | 0% |
| Kosakonia arachidis | 100% | 0% | 100% | 0% |
| Leclercia adecarboxylata | 100% | 0% | 90.48% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0.02% | 0% |
| Raoultella planticola | 100% | 0% | 94.12% | 0% |
| Salmonella enterica | 94.37% | 0% | 88.71% | 0% |
| Shigella boydii | 92.86% | 0% | 91.4% | 0% |
| Shigella dysenteriae | 90.91% | 0% | 89.29% | 0% |
| Shigella flexneri | 100% | 0% | 83.33% | 0% |
| Shigella sonnei | 100% | 0% | 94.37% | 0% |
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| No prevalence data | ||||
Model Type: protein overexpression model
Model Definition: This model detects protein overexpression based on the presence of mutations. The detection of the protein without an associated mutation indicates that the protein is likely to be expressed at low or basal levels. The detection of the protein with the mutation indicates that the protein is likely overexpressed. This model reflects how certain proteins are functional with and without mutations. For example, efflux pump subunits and regulators are functional with mutations and without mutations. Without mutations, efflux pump subunits and regulators are usually expressed at a low level. When an efflux pump regulator has a mutation, it can cause the overexpression of the efflux pump it is responsible for regulating, leading to resistance to specific drugs. Protein overexpression models have two parameters: a curated BLASTP cutoff, and a curated set of mutations (single resistance variants, frameshift mutations, indels, etc.) shown clinically to confer resistance. This model type is a combination of the protein homolog and protein variant model. A detected hit can be categorized as Perfect, Strict, or Loose with no mutation(s) or as Strict or Loose with mutation(s).
Bit-score Cut-off (blastP): 210
Legend:
Published Variants:
PMID in progress (single): V45E A70T R73S R73C R77L R77C Y137H
| PMID: 9687412 | E31STOP I49S L78M R94H R94S |
| PMID: 8807064 | S3N V96E G103S |
| PMID: 8550435 | R58L |
Model Name: Escherichia coli AcrAB-TolC with MarR mutations conferring resistance to ciprofloxacin and tetracycline
Model Description: The Escherichia coli AcrAB-TolC with MarR mutation (Y137H) conferring resistance to ciprofloxacin and tetracycline.
Escherichia coli marR mutant conferring antibiotic resistanceTolCacrBEscherichia coli acrA