Enterobacter cloacae acrA

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Accession ARO:3004042
CARD Short NameEclo_acrA
DefinitionAcrA is a subunit of the AcrAB-TolC multidrug efflux system in E. cloacae.
AMR Gene Familyresistance-nodulation-cell division (RND) antibiotic efflux pump
Drug Classtetracycline antibiotic, penam, disinfecting agents and antiseptics, phenicol antibiotic, rifamycin antibiotic, glycylcycline, cephalosporin, fluoroquinolone antibiotic
Resistance Mechanismantibiotic efflux
Efflux Componentefflux pump complex or subunit conferring antibiotic resistance
Resistomes with Perfect MatchesEnterobacter cloacaeg+wgs, Enterobacter roggenkampiig+wgs
Resistomes with Sequence VariantsEnterobacter cloacaeg+wgs, Enterobacter roggenkampiig+wgs
Classification26 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ acrA
+ confers_resistance_to_antibiotic tigecycline [Antibiotic]
Publications

Keeney D, et al. 2007. Microb Drug Resist 13(1): 1-6. RamA, a transcriptional regulator, and AcrAB, an RND-type efflux pump, are associated with decreased susceptibility to tigecycline in Enterobacter cloacae. (PMID 17536927)

Pradel E and Pages JM. 2002. Antimicrob Agents Chemother 46(8): 2640-2643. The AcrAB-TolC efflux pump contributes to multidrug resistance in the nosocomial pathogen Enterobacter aerogenes. (PMID 12121946)

Resistomes

Prevalence of Enterobacter cloacae acrA among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Enterobacter cloacae3.57%0%1.6%0%
Enterobacter roggenkampii81.4%0%51.44%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 760


>gb|ABG77965.1|+|Enterobacter cloacae acrA [Enterobacter cloacae]
MNKNRGLTPLAVVLMLSGSLALTGCDDKPAQQGAQQAPEVGVVTLKSEPLQITTELPGRTNAYRIAEVRPQVSGIILKRNFTEGGDVQAG
ESLYQIDPATYQASYESAKGDLAKAQAAAKIAQLTLNRYQKLLGTKYISQQDYDTALADAQQANAAVVAAKAAVETARINLAYTKVTSPI
SGRIGKSSVTEGALVQNGQTTALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENGKAKVELITNDGIKFPQEGTLEFSDVTVDQT
TGSITLRAIFPNPDKNLLPGMFVRARLEEGTNPTALLVPQQGVTRTPRGDASALVVGADNKVEMRNITATQAIGDKWLVTEGLKDGDRVI
VTGLQKVRPGAQVKAQEVKSDDKQQASAAGQSEQTKS


>gb|DQ679966.1|+|300-1493|Enterobacter cloacae acrA [Enterobacter cloacae]
ATGAACAAAAACAGAGGGTTAACGCCTCTGGCGGTCGTTCTGATGCTTTCAGGCAGCTTAGCGCTAACAGGATGTGACGACAAACCGGCT
CAACAAGGAGCTCAGCAGGCGCCAGAAGTAGGCGTTGTGACGCTCAAATCTGAACCTCTACAAATCACCACCGAATTACCCGGCCGTACA
AATGCTTACCGCATTGCGGAAGTGCGTCCTCAGGTTAGCGGCATTATCCTGAAACGCAACTTCACCGAAGGCGGTGATGTGCAGGCCGGT
GAGTCTCTGTATCAGATTGATCCCGCAACCTATCAGGCGTCTTATGAAAGCGCGAAAGGCGATCTGGCTAAAGCGCAGGCCGCGGCTAAA
ATTGCCCAGCTGACGCTGAACCGCTATCAAAAACTGCTCGGTACCAAGTACATCAGTCAGCAGGATTACGATACCGCCCTGGCGGATGCC
CAGCAGGCTAACGCCGCCGTGGTGGCAGCCAAAGCGGCCGTCGAAACCGCGCGCATTAACCTGGCCTATACCAAAGTGACCTCCCCTATC
AGCGGTCGTATTGGTAAATCTTCCGTCACGGAAGGGGCTCTGGTGCAAAACGGTCAGACCACTGCGCTGGCGACCGTGCAGCAGCTCGAT
CCGATCTATGTTGACGTCACGCAGTCCAGCAATGATTTCCTGCGCCTGAAACAGGAGTTGGCTAACGGCACCCTGAAACAGGAAAACGGC
AAAGCCAAAGTGGAGCTGATTACCAACGACGGTATCAAGTTCCCGCAGGAAGGGACGCTGGAATTCTCTGACGTGACGGTCGACCAGACC
ACCGGTTCCATCACCTTACGTGCGATTTTCCCGAACCCTGACAAAAATCTGCTGCCAGGTATGTTCGTTCGCGCACGTCTGGAAGAAGGA
ACGAATCCAACCGCCCTTCTGGTTCCACAGCAGGGTGTGACCCGTACGCCACGCGGCGATGCGAGCGCACTGGTTGTTGGGGCTGATAAC
AAAGTCGAAATGCGCAACATCACCGCCACCCAGGCGATTGGGGATAAATGGCTGGTGACGGAAGGTCTGAAAGATGGCGATCGCGTGATT
GTTACTGGTTTGCAAAAAGTTCGTCCTGGCGCGCAGGTTAAAGCACAGGAAGTGAAATCTGACGATAAACAACAAGCTTCGGCCGCTGGC
CAGTCAGAACAAACCAAGTCTTAA