OprJ

Accession ARO:3000802
DefinitionOprJ is the outer membrane channel component of the MexCD-OprJ multidrug efflux complex.
AMR Gene Familyresistance-nodulation-cell division (RND) antibiotic efflux pump
Drug Classtetracycline antibiotic, diaminopyrimidine antibiotic, phenicol antibiotic, monobactam, antibacterial free fatty acids, acridine dye, glycylcycline, penam, carbapenem, triclosan, fluoroquinolone antibiotic, aminoglycoside antibiotic, aminocoumarin antibiotic, macrolide antibiotic, cephalosporin
Resistance Mechanismantibiotic efflux
Efflux Componentefflux pump complex or subunit conferring antibiotic resistance
Classification34 ontology terms | Show
Parent Term(s)4 ontology terms | Show
Publications

Srikumar R, et al. 1998. Antimicrob Agents Chemother 42(1): 65-71. Expression of Pseudomonas aeruginosa multidrug efflux pumps MexA-MexB-OprM and MexC-MexD-OprJ in a multidrug-sensitive Escherichia coli strain. (PMID 9449262)

Resistomes

Prevalence of OprJ among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
Klebsiella pneumoniae0%0%0%
Pseudomonas aeruginosa0%0%0%
Pseudomonas fluorescens3.7%0%0%
Staphylococcus aureus0%0%0.01%
Vibrio vulnificus0%0%0.68%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 850


>gb|AAB41958.1|+|OprJ [Pseudomonas aeruginosa]
MRKPAFGVSALLIALTLGACSMAPTYERPAAPVADSWSGAAAQRQGAAIDTLDWKSFIVDAELRRLVDMALDNIRSLRQTLLDIEAARAQ
YRIQRADRVPGLNAAATGNRQRQPADLSAGNRSEVASSYQVGLALPEYELDLFGRVKSLTDAALQQYLASEEAARAARIALVAEVSQAYL
SYDGALRRLALTRQTLVSREYSFALIDQRRAAGAATALDYQEALGLVEQARAEQERNLRQKQQAFNALVLLLGSDDAAQAIPRSPGQRPK
LLQDIAPGTPSELIERRPDILAAEHRLRARNADIGAARAAFFPRISLTGSFGTSSAEMSGLFDGGSRSCSFLPTLTLPIFDGGRNRANLS
LAEARKDSAVAAYEGTIQTAFREVADALAASDTLRREEKALRALANSSNEALKLAKARYESGVDNHLRYLDAQRSSFLNEIAFIDGSTQR
QIALVDLFRALGGGWDEGRSLVVHRGGRS


>gb|U57969|+|4623-6062|OprJ [Pseudomonas aeruginosa]
ATGCGCAAACCTGCTTTCGGCGTATCGGCGCTGCTGATCGCCCTGACCCTCGGCGCCTGCTCCATGGCGCCGACCTACGAACGTCCCGCC
GCGCCGGTGGCCGACAGCTGGAGCGGCGCCGCCGCCCAGCGCCAGGGCGCGGCGATCGACACGCTGGATTGGAAGAGTTTCATCGTCGAT
GCCGAACTACGCCGCCTGGTGGACATGGCCCTGGATAACATCCGCTCGCTGCGCCAGACCCTCCTGGATATCGAGGCGGCCCGCGCGCAG
TACCGAATCCAGCGCGCCGACCGGGTTCCGGGCCTGAATGCCGCTGCCACCGGCAACCGCCAGCGGCAGCCGGCCGACCTGTCCGCCGGC
AATCGCTCGGAAGTGGCCAGCAGCTACCAGGTCGGGCTGGCCCTGCCGGAGTACGAACTGGACCTCTTCGGTCGGGTCAAGAGCCTGACC
GACGCAGCCCTGCAACAGTACCTGGCCAGCGAGGAGGCAGCGCGCGCGGCACGGATCGCCCTGGTCGCCGAGGTCAGCCAGGCCTACCTG
AGCTACGACGGCGCCCTGCGGCGCCTGGCGCTGACCCGTCAGACCCTGGTCAGCCGCGAGTATTCCTTCGCCCTGATCGACCAGCGCCGC
GCGGCCGGCGCCGCCACCGCGCTGGACTACCAGGAAGCCCTTGGCCTGGTGGAGCAGGCGCGCGCCGAGCAGGAGCGCAACCTGCGGCAG
AAACAGCAGGCATTCAACGCGCTGGTGTTGCTGCTGGGTAGCGACGATGCCGCGCAGGCGATTCCGCGGAGTCCGGGGCAGCGGCCGAAG
CTGCTGCAGGACATCGCTCCCGGCACGCCGTCCGAGCTGATCGAGCGACGTCCGGACATCCTTGCCGCCGAACATCGTTTGCGGGCGCGC
AACGCGGATATCGGCGCGGCGCGCGCGGCGTTCTTCCCGCGCATCAGCCTGACCGGCAGCTTCGGCACCTCCAGCGCGGAAATGTCCGGC
CTGTTCGATGGCGGCTCGCGCTCCTGTAGCTTCCTGCCGACGTTGACGCTGCCGATCTTCGATGGCGGGCGCAACCGTGCCAACCTGAGC
CTGGCCGAGGCGCGCAAGGATTCGGCGGTGGCCGCCTACGAGGGGACCATCCAGACCGCTTTCCGCGAGGTCGCCGACGCCCTGGCGGCC
AGCGATACCCTGCGTCGCGAAGAGAAGGCCCTGCGCGCCCTGGCGAACAGCAGCAACGAAGCCCTGAAGCTGGCCAAGGCACGCTACGAG
AGTGGCGTCGACAACCACCTGCGCTACCTCGATGCGCAGCGCAGCAGCTTCCTCAACGAGATCGCCTTCATCGACGGCAGCACCCAGCGG
CAGATCGCCCTGGTCGACCTGTTCCGCGCGCTCGGCGGAGGCTGGGACGAGGGACGGAGCCTGGTGGTACATCGAGGCGGCAGGAGTTGA