| Accession | ARO:3000379 |
| CARD Short Name | OprM |
| Definition | OprM is an outer membrane factor protein found in Pseudomonas aeruginosa and Burkholderia vietnamiensis. It is part of the MexAB-OprM, MexVW-OprM, MexXY-OprM and the AmrAB-OprM complex. |
| AMR Gene Family | resistance-nodulation-cell division (RND) antibiotic efflux pump |
| Drug Class | peptide antibiotic, penam, diaminopyrimidine antibiotic, sulfonamide antibiotic, cephalosporin, carbapenem, macrolide antibiotic, tetracycline antibiotic, monobactam, phenicol antibiotic, aminocoumarin antibiotic, fluoroquinolone antibiotic, cephamycin, penem, disinfecting agents and antiseptics, aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Perfect Matches | Pseudomonas aeruginosag+p+wgs, Pseudomonas fluorescensg |
| Resistomes with Sequence Variants | Acinetobacter baumanniiwgs, Burkholderia multivoranswgs, Enterobacter cloacaewgs, Escherichia coliwgs, Klebsiella pneumoniaewgs, Pseudomonas aeruginosag+p+wgs, Pseudomonas fluorescensg, Stenotrophomonas maltophiliawgs |
| Classification | 63 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + resistance-modifying agents + beta-lactamase inhibitor + peptide antibiotic [Drug Class] + lipopeptide antibiotic + beta-lactam antibiotic + serine beta-lactamase inhibitor + mechanism of antibiotic resistance + polymyxin antibiotic + cephem + penam [Drug Class] + determinant of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + diaminopyrimidine antibiotic [Drug Class] + sulfonamide antibiotic [Drug Class] + beta-lactam derived beta-lactamase inhibitor + sulfamethoxazole [Antibiotic] + amoxicillin [Antibiotic] + cephalosporin [Drug Class] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + colistin + antibiotic mixture + carbapenem [Drug Class] + macrolide antibiotic [Drug Class] + tetracycline antibiotic [Drug Class] + monobactam [Drug Class] + phenicol antibiotic [Drug Class] + aminocoumarin antibiotic [Drug Class] + trimethoprim [Antibiotic] + clavulanic acid [Adjuvant] + fluoroquinolone antibiotic [Drug Class] + ciprofloxacin [Antibiotic] + colistin A [Antibiotic] + amoxicillin-clavulanic acid [Antibiotic+Adjuvant] + ceftriaxone [Antibiotic] + cephamycin [Drug Class] + novobiocin [Antibiotic] + trimethoprim-sulfamethoxazole [Antibiotic] + colistin B [Antibiotic] + tetracycline [Antibiotic] + ceftazidime [Antibiotic] + azithromycin [Antibiotic] + resistance-nodulation-cell division (RND) antibiotic efflux pump [AMR Gene Family] + aztreonam [Antibiotic] + penem [Drug Class] + chloramphenicol [Antibiotic] + erythromycin [Antibiotic] + panipenem [Antibiotic] + meropenem [Antibiotic] + disinfecting agents and antiseptics [Drug Class] + ampicillin [Antibiotic] + aminoglycoside antibiotic [Drug Class] + nalidixic acid [Antibiotic] + ticarcillin [Antibiotic] + MexAB-OprM + amikacin [Antibiotic] + thiamphenicol [Antibiotic] + norfloxacin [Antibiotic] + tobramycin [Antibiotic] + gentamicin C [Antibiotic] + ofloxacin [Antibiotic] + acriflavine [Antibiotic] |
| Parent Term(s) | 12 ontology terms | Show + part_of MexAB-OprM + subunit of efflux pump conferring antibiotic resistance + part_of MexXY-OprM + part_of AmrAB-OprM + part_of MexVW-OprM + part_of MexJK-OprM + part_of MexMN-OprM + part_of MexAB-OprM with prematurely terminated MexR conferring resistance to meropenem and ciprofloxacin + part_of MexAB-OprM with NalC mutations conferring resistance to aztreonam + part_of MexAB-OprM with NalD mutations conferring resistance to multiple antibiotics + part_of MexAB-OprM with CpxR regulator conferring resistance to ciprofloxacin, ceftazidime, and aztreonam + part_of MexAB-OprM with MexR mutations confers resistance to multiple antibiotics |
| Publications | Lambert O, et al. 2005. J Struct Biol 150(1): 50-57. Trimeric structure of OprN and OprM efflux proteins from Pseudomonas aeruginosa, by 2D electron crystallography. (PMID 15797729) Akama H, et al. 2005. Acta Crystallogr Sect F Struct Biol Cryst Commun 61(PT 1): 131-133. Preliminary crystallographic analysis of the antibiotic discharge outer membrane lipoprotein OprM of Pseudomonas aeruginosa with an exceptionally long unit cell and complex lattice structure. (PMID 16508113) Broutin I, et al. 2005. Acta Crystallogr Sect F Struct Biol Cryst Commun 61(PT 3): 315-318. Expression, purification, crystallization and preliminary X-ray studies of the outer membrane efflux proteins OprM and OprN from Pseudomonas aeruginosa. (PMID 16511029) |
Prevalence of OprM among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 0% | 0% | 0.02% | 0% |
| Burkholderia multivorans | 0% | 0% | 0.32% | 0% |
| Enterobacter cloacae | 0% | 0% | 0.36% | 0% |
| Escherichia coli | 0% | 0% | 0.01% | 0% |
| Klebsiella pneumoniae | 0% | 0% | 0.01% | 0% |
| Pseudomonas aeruginosa | 100% | 0.64% | 74.9% | 0% |
| Pseudomonas fluorescens | 2.86% | 0% | 0% | 0% |
| Stenotrophomonas maltophilia | 0% | 0% | 0.34% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 850
